Document Detail


K-fibre minus ends are stabilized by a RanGTP-dependent mechanism essential for functional spindle assembly.
MedLine Citation:
PMID:  22081094     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromosome segregation requires the formation of K-fibres, microtubule bundles that attach sister kinetochores to spindle poles. Most K-fibre microtubules originate around the chromosomes through a non-centrosomal RanGTP-dependent pathway and become oriented with the plus ends attached to the kinetochore and the minus ends focused at the spindle poles. The capture and stabilization of microtubule plus ends at the kinetochore has been extensively studied but very little is known on how their minus-end dynamics are controlled. Here we show that MCRS1 is a RanGTP-regulated factor essential for non-centrosomal microtubule assembly. MCRS1 localizes to the minus ends of chromosomal microtubules and K-fibres, where it protects them from depolymerization. Our data reveal the existence of a mechanism that stabilizes the minus ends of chromosomal microtubules and K-fibres, and is essential for the assembly of a functional bipolar spindle.
Authors:
Sylvain Meunier; Isabelle Vernos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-11-13
Journal Detail:
Title:  Nature cell biology     Volume:  13     ISSN:  1476-4679     ISO Abbreviation:  Nat. Cell Biol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-02     Completed Date:  2012-01-26     Revised Date:  2012-02-02    
Medline Journal Info:
Nlm Unique ID:  100890575     Medline TA:  Nat Cell Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1406-14     Citation Subset:  IM    
Affiliation:
Cell and Developmental Biology Program, Centre for Genomic Regulation, Universitat Pompeu Fabra, 08003 Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle Proteins / genetics,  metabolism
Chromosome Segregation / physiology*
HeLa Cells
Humans
Kinesin / genetics,  metabolism
Kinetochores / physiology*
Microtubule-Associated Proteins / genetics,  metabolism
Microtubules / metabolism
Mitosis / physiology
Mitotic Spindle Apparatus / metabolism*
Nuclear Proteins / genetics,  metabolism*
Oocytes
RNA-Binding Proteins / genetics,  metabolism*
Xenopus
beta Karyopherins / metabolism
ran GTP-Binding Protein / physiology*
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/KIF2C protein, human; 0/MCRS1 protein, human; 0/Microtubule-Associated Proteins; 0/Nuclear Proteins; 0/RAN protein, human; 0/RNA-Binding Proteins; 0/TPX2 protein, human; 0/beta Karyopherins; EC 3.6.1.-/Kinesin; EC 3.6.5.2/ran GTP-Binding Protein
Comments/Corrections
Comment In:
Nat Rev Mol Cell Biol. 2011;13(1):2-3   [PMID:  22166991 ]
Nat Cell Biol. 2011 Dec;13(12):1389-91   [PMID:  22081093 ]

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