Document Detail

K-fibre minus ends are stabilized by a RanGTP-dependent mechanism essential for functional spindle assembly.
MedLine Citation:
PMID:  22081094     Owner:  NLM     Status:  MEDLINE    
Chromosome segregation requires the formation of K-fibres, microtubule bundles that attach sister kinetochores to spindle poles. Most K-fibre microtubules originate around the chromosomes through a non-centrosomal RanGTP-dependent pathway and become oriented with the plus ends attached to the kinetochore and the minus ends focused at the spindle poles. The capture and stabilization of microtubule plus ends at the kinetochore has been extensively studied but very little is known on how their minus-end dynamics are controlled. Here we show that MCRS1 is a RanGTP-regulated factor essential for non-centrosomal microtubule assembly. MCRS1 localizes to the minus ends of chromosomal microtubules and K-fibres, where it protects them from depolymerization. Our data reveal the existence of a mechanism that stabilizes the minus ends of chromosomal microtubules and K-fibres, and is essential for the assembly of a functional bipolar spindle.
Sylvain Meunier; Isabelle Vernos
Related Documents :
19060894 - Genome stability is ensured by temporal control of kinetochore-microtubule dynamics.
17036054 - Microtubule depolymerization can drive poleward chromosome motion in fission yeast.
12426374 - Spindle-kinetochore attachment requires the combined action of kin i-like klp5/6 and al...
16219694 - Silencing cenp-f weakens centromeric cohesion, prevents chromosome alignment and activa...
1314694 - Allelotype of non-small cell lung carcinoma--comparison between loss of heterozygosity ...
3100504 - Cloning of the reca gene of neisseria gonorrhoeae and construction of gonococcal reca m...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-11-13
Journal Detail:
Title:  Nature cell biology     Volume:  13     ISSN:  1476-4679     ISO Abbreviation:  Nat. Cell Biol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-02     Completed Date:  2012-01-26     Revised Date:  2012-02-02    
Medline Journal Info:
Nlm Unique ID:  100890575     Medline TA:  Nat Cell Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1406-14     Citation Subset:  IM    
Cell and Developmental Biology Program, Centre for Genomic Regulation, Universitat Pompeu Fabra, 08003 Barcelona, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Cycle Proteins / genetics,  metabolism
Chromosome Segregation / physiology*
HeLa Cells
Kinesin / genetics,  metabolism
Kinetochores / physiology*
Microtubule-Associated Proteins / genetics,  metabolism
Microtubules / metabolism
Mitosis / physiology
Mitotic Spindle Apparatus / metabolism*
Nuclear Proteins / genetics,  metabolism*
RNA-Binding Proteins / genetics,  metabolism*
beta Karyopherins / metabolism
ran GTP-Binding Protein / physiology*
Reg. No./Substance:
0/Cell Cycle Proteins; 0/KIF2C protein, human; 0/MCRS1 protein, human; 0/Microtubule-Associated Proteins; 0/Nuclear Proteins; 0/RAN protein, human; 0/RNA-Binding Proteins; 0/TPX2 protein, human; 0/beta Karyopherins; EC 3.6.1.-/Kinesin; EC GTP-Binding Protein
Comment In:
Nat Rev Mol Cell Biol. 2011;13(1):2-3   [PMID:  22166991 ]
Nat Cell Biol. 2011 Dec;13(12):1389-91   [PMID:  22081093 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A new cap for kinetochore fibre minus ends.
Next Document:  Transarterial endovascular treatment in the management of life-threatening intra- and postoperative ...