| K depletion stimulates in vivo HCO3 reabsorption in surviving rat distal tubules. | |
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MedLine Citation:
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PMID: 9575889 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To evaluate whether K depletion enhances in vivo bicarbonate reabsorption (JtCO2) in surviving distal tubules (DT), we compared DT JtCO2 in five-sixths nephrectomized rats (Nx) with and without dietary K depletion (Nx-K). Furthermore, to identify possible mechanisms of increased JtCO2, we perfused inhibitors of proton secretion in both Nx and Nx-K rats. JtCO2 (102 +/- 8 pmol.min-1.mm-1) was significantly increased in Nx-K vs. Nx rats (65 +/- 7 pmol.min-1.mm-1, P < 0.05) but unaffected by 10(-6) M losartan perfusion (94 +/- 6 pmol.min-1.mm-1, P = not significant). Although 10(-5) M Sch-28080 also had no significant effect, 5 x 10(-9) M concanamycin A perfusion significantly decreased JtCO2 in Nx-K rats to 65 +/- 8 pmol.min-1. mm-1 (P < 0.05). Morphometric evaluation and H(+)-ATPase immunogold labeling of Nx-K A-type intercalated cells revealed cellular hypertrophy, elaborated apical microplicae, and enhanced H(+)-ATPase apical polarization. Accordingly, these combined studies confirm that K depletion enhances JtCO2 in surviving DT by stimulating H(+)-ATPase activity, independent of the AT1 receptor. |
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Authors:
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D Z Levine; M Iacovitti; S Buckman; B Luck; M T Hincke; K D Burns; J N Fryer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 274 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1998 Apr |
Date Detail:
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Created Date: 1998-06-02 Completed Date: 1998-06-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: F665-72 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Ottawa, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Absorption
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physiology Animals Anti-Bacterial Agents / pharmacology Bicarbonates / metabolism* Blotting, Western Enzyme Inhibitors / pharmacology H(+)-K(+)-Exchanging ATPase / metabolism Imidazoles / pharmacology Immunochemistry Immunohistochemistry Kidney Tubules, Distal / metabolism* Macrolides* Male Microscopy, Electron Nephrectomy Potassium Deficiency / metabolism* Rats Rats, Sprague-Dawley Stomach / enzymology |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Bicarbonates; 0/Enzyme Inhibitors; 0/Imidazoles; 0/Macrolides; 76081-98-6/Sch 28080; 80890-47-7/concanamycin A; EC 3.6.1.10/H(+)-K(+)-Exchanging ATPase |
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