Document Detail

K+ channels and the microglial respiratory burst.
MedLine Citation:
PMID:  11245596     Owner:  NLM     Status:  MEDLINE    
Microglial activation following central nervous system damage or disease often culminates in a respiratory burst that is necessary for antimicrobial function, but, paradoxically, can damage bystander cells. We show that several K+ channels are expressed and play a role in the respiratory burst of cultured rat microglia. Three pharmacologically separable K+ currents had properties of Kv1.3 and the Ca2+/calmodulin-gated channels, SK2, SK3, and SK4. mRNA was detected for Kv1.3, Kv1.5, SK2, and/or SK3, and SK4. Protein was detected for Kv1.3, Kv1.5, and SK3 (selective SK2 and SK4 antibodies not available). No Kv1.5-like current was detected, and confocal immunofluorescence showed the protein to be subcellular, in contrast to the robust membrane localization of Kv1.3. To determine whether any of these channels play a role in microglial activation, a respiratory burst was stimulated with phorbol 12-myristate 13-acetate and measured using a single cell, fluorescence-based dihydrorhodamine 123 assay. The respiratory burst was markedly inhibited by blockers of SK2 (apamin) and SK4 channels (clotrimazole and charybdotoxin), and to a lesser extent, by the potent Kv1.3 blocker agitoxin-2.
R Khanna; L Roy; X Zhu; L C Schlichter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  280     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-03-14     Completed Date:  2001-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C796-806     Citation Subset:  IM    
Division of Cellular and Molecular Biology, Toronto Western Research Institute, University Health Network, Toronto, Ontario, Canada.
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MeSH Terms
Apamin / pharmacology
Cells, Cultured
Charybdotoxin / pharmacology
Clotrimazole / pharmacology
Gene Expression / physiology
Growth Inhibitors / pharmacology
Intermediate-Conductance Calcium-Activated Potassium Channels
Ion Channel Gating / physiology
Kv1.3 Potassium Channel
Kv1.5 Potassium Channel
Membrane Potentials / physiology
Microglia / cytology,  metabolism*
NADP / metabolism
Patch-Clamp Techniques
Potassium Channel Blockers
Potassium Channels / genetics,  metabolism*
Potassium Channels, Calcium-Activated*
Potassium Channels, Voltage-Gated*
Rats, Wistar
Reactive Oxygen Species / metabolism
Respiratory Burst / physiology*
Scorpion Venoms / pharmacology
Small-Conductance Calcium-Activated Potassium Channels
Reg. No./Substance:
0/Growth Inhibitors; 0/Intermediate-Conductance Calcium-Activated Potassium Channels; 0/Kcna3 protein, rat; 0/Kcna5 protein, rat; 0/Kcnn2 protein, rat; 0/Kcnn3 protein, rat; 0/Kcnn4 protein, rat; 0/Kv1.3 Potassium Channel; 0/Kv1.5 Potassium Channel; 0/Potassium Channel Blockers; 0/Potassium Channels; 0/Potassium Channels, Calcium-Activated; 0/Potassium Channels, Voltage-Gated; 0/Reactive Oxygen Species; 0/Scorpion Venoms; 0/Small-Conductance Calcium-Activated Potassium Channels; 115422-61-2/Charybdotoxin; 155646-22-3/agitoxin 2; 23593-75-1/Clotrimazole; 24345-16-2/Apamin; 53-59-8/NADP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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