| K+ channel expression in primary cell cultures mediated by vaccinia virus. | |
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MedLine Citation:
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PMID: 1899389 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A recombinant vaccinia virus (VV) was used to express functional Drosophila Shaker H4 K+ channels in primary cell cultures from rat heart (atrial and ventricular myocytes, fibroblasts), autonomic ganglia (SCG neurons) and CNS (hippocampal neurons, cerebral astroglia). In most cells the expressed currents possessed the typical characteristics of the native Drosophila muscle A currents; a few cells showed evidence of hetero-oligomers with new properties. The maximum current density corresponded to a channel density of 2-3/microns 2. Voltage recordings in heart cells showed altered action potential waveforms after successful infection. VV vectors thus are useful for studying altered excitability and cell-specific processing of ion channel proteins. |
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Authors:
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A Karschin; J Aiyar; A Gouin; N Davidson; H A Lester |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: FEBS letters Volume: 278 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1991 Jan |
Date Detail:
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Created Date: 1991-03-13 Completed Date: 1991-03-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 229-33 Citation Subset: IM |
Affiliation:
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Division of Biology 156-29, California Institute of Technology, Pasadena 91125. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Action Potentials Animals Cells, Cultured Drosophila melanogaster / genetics Electric Conductivity Gene Expression Genetic Vectors Hippocampus / cytology, physiology Muscles / physiology Potassium Channels / genetics*, physiology Rats Recombinant Proteins Vaccinia virus / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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GM-10991/GM/NIGMS NIH HHS; GM-29836/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Potassium Channels; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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