| Juvenile hormone biosynthesis by corpora allata of larval tomato moth, Lacanobia oleracea, and regulation by Manduca sexta allatostatin and allatotropin. | |
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MedLine Citation:
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PMID: 10876111 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Juvenile hormone (JH) biosynthesis and the effects of synthetic Manduca sexta allatostatin (Mas-AS) and M. sexta allatotropin (Mas-AT) were investigated in isolated corpora allata (CA) of Vth stadium larvae of the tomato moth, Lacanobia oleracea. Reversed-phase high-performance liquid chromatography (RP-HPLC) of JH extracted from CA shows that larvae produce predominantly JH II and its corresponding acid. It appears that the acid homologue is a result of JH esterase activity in the CA (and other tissues) rather than the lack of JH acid methyltransferase. Mean rates of synthesis (100-200fmol/pr/h) were inhibited ca. 70% by Mas-AS and stimulated in a dose-dependent manner up to three times by Mas-AT. However, Mas-AS had no significant effect on Mas-AT-stimulated rates of JH biosynthesis. Using RP-HPLC and an enzyme-linked immunosorbent assay (ELISA) to Mas-AT, a peak of Mas-AT-like immunoreactivity was detected in larval L. oleracea brain homogenates. Co-elution of this immunoreactive peak with synthetic Mas-AT suggests that this neuropeptide is also present in L. oleracea. |
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Authors:
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N Audsley; R J Weaver; J P Edwards |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Insect biochemistry and molecular biology Volume: 30 ISSN: 0965-1748 ISO Abbreviation: Insect Biochem. Mol. Biol. Publication Date: 2000 Aug-Sep |
Date Detail:
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Created Date: 2000-11-07 Completed Date: 2000-11-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9207282 Medline TA: Insect Biochem Mol Biol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 681-9 Citation Subset: IM |
Affiliation:
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Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK. n.audsley@csl.gov.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Brain / metabolism Carboxylic Ester Hydrolases / metabolism Corpora Allata / metabolism* Hormone Antagonists / metabolism* Insect Hormones / metabolism* Juvenile Hormones / biosynthesis* Larva / metabolism Lycopersicon esculentum Manduca / metabolism Molecular Sequence Data Moths / metabolism Neuropeptides / metabolism* Tissue Extracts / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Hormone Antagonists; 0/Insect Hormones; 0/Juvenile Hormones; 0/Neuropeptides; 0/Tissue Extracts; 110119-33-0/allatostatin; 75831-28-6/allatotropin; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.-/juvenile hormone esterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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