Document Detail

Juvenile hormone biosynthesis by corpora allata of larval tomato moth, Lacanobia oleracea, and regulation by Manduca sexta allatostatin and allatotropin.
MedLine Citation:
PMID:  10876111     Owner:  NLM     Status:  MEDLINE    
Juvenile hormone (JH) biosynthesis and the effects of synthetic Manduca sexta allatostatin (Mas-AS) and M. sexta allatotropin (Mas-AT) were investigated in isolated corpora allata (CA) of Vth stadium larvae of the tomato moth, Lacanobia oleracea. Reversed-phase high-performance liquid chromatography (RP-HPLC) of JH extracted from CA shows that larvae produce predominantly JH II and its corresponding acid. It appears that the acid homologue is a result of JH esterase activity in the CA (and other tissues) rather than the lack of JH acid methyltransferase. Mean rates of synthesis (100-200fmol/pr/h) were inhibited ca. 70% by Mas-AS and stimulated in a dose-dependent manner up to three times by Mas-AT. However, Mas-AS had no significant effect on Mas-AT-stimulated rates of JH biosynthesis. Using RP-HPLC and an enzyme-linked immunosorbent assay (ELISA) to Mas-AT, a peak of Mas-AT-like immunoreactivity was detected in larval L. oleracea brain homogenates. Co-elution of this immunoreactive peak with synthetic Mas-AT suggests that this neuropeptide is also present in L. oleracea.
N Audsley; R J Weaver; J P Edwards
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Insect biochemistry and molecular biology     Volume:  30     ISSN:  0965-1748     ISO Abbreviation:  Insect Biochem. Mol. Biol.     Publication Date:    2000 Aug-Sep
Date Detail:
Created Date:  2000-11-07     Completed Date:  2000-11-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9207282     Medline TA:  Insect Biochem Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  681-9     Citation Subset:  IM    
Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK.
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MeSH Terms
Amino Acid Sequence
Brain / metabolism
Carboxylic Ester Hydrolases / metabolism
Corpora Allata / metabolism*
Hormone Antagonists / metabolism*
Insect Hormones / metabolism*
Juvenile Hormones / biosynthesis*
Larva / metabolism
Lycopersicon esculentum
Manduca / metabolism
Molecular Sequence Data
Moths / metabolism
Neuropeptides / metabolism*
Tissue Extracts / metabolism
Reg. No./Substance:
0/Hormone Antagonists; 0/Insect Hormones; 0/Juvenile Hormones; 0/Neuropeptides; 0/Tissue Extracts; 110119-33-0/allatostatin; 75831-28-6/allatotropin; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.-/juvenile hormone esterase

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