| Junction protein shrew-1 influences cell invasion and interacts with invasion-promoting protein CD147. | |
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MedLine Citation:
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PMID: 17267690 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Shrew-1 was previously isolated from an endometriotic cell line in our search for invasion-associated genes. It proved to be a membrane protein that targets to the basolateral membrane of polarized epithelial cells, interacting with E-cadherin-catenin complexes of adherens junctions. Paradoxically, the existence of adherens junctions is incompatible with invasion. To investigate whether shrew-1 can indeed influence cellular invasion, we overexpressed it in HT1080 fibrosarcoma cells. This resulted in enhanced invasiveness, accompanied by an increased matrix metalloprotease (MMP)-9 level in the supernatant, raising the question about the role of shrew-1 in this process. Logic suggested we looked for an interaction with CD147, a known promoter of invasiveness and MMP activity. Indeed, genetics-based, biochemical, and microscopy experiments revealed shrew-1- and CD147-containing complexes in invasive endometriotic cells and an interaction in epithelial cells, which was stronger in MCF7 tumor cells, but weaker in Madin-Darby canine kidney cells. In contrast to the effect mediated by overexpression, small interfering RNA-mediated down-regulation of either shrew-1 or CD147 in HeLa cells decreased invasiveness without affecting the proliferation behavior of HeLa cells, but the knockdown cells displayed decreased motility. Altogether, our results imply that shrew-1 has a function in the regulation of cellular invasion, which may involve its interaction with CD147. |
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Authors:
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Alexander Schreiner; Mika Ruonala; Viktor Jakob; Jan Suthaus; Eckhard Boles; Fred Wouters; Anna Starzinski-Powitz |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-31 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 18 ISSN: 1059-1524 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-03-29 Completed Date: 2007-06-04 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 1272-81 Citation Subset: IM |
Affiliation:
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Institute of Cell Biology and Neuroscience, Johann Wolfgang Goethe University of Frankfurt, D-60323 Frankfurt am Main, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD147 / genetics, metabolism* Base Sequence Cell Adhesion Molecules Cell Movement* Cells, Cultured Dogs Endometriosis / pathology Female Humans Immunoprecipitation Matrix Metalloproteinase 2 / metabolism Matrix Metalloproteinase 9 / metabolism Membrane Proteins / genetics, metabolism* Microscopy, Fluorescence / methods Molecular Sequence Data Neoplasm Invasiveness Peptide Fragments / metabolism RNA, Small Interfering Ubiquitin / metabolism Yeasts / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/AJAP1 protein, human; 0/BSG protein, human; 0/Cell Adhesion Molecules; 0/Membrane Proteins; 0/Peptide Fragments; 0/RNA, Small Interfering; 0/Ubiquitin; 136894-56-9/Antigens, CD147; EC 3.4.24.24/Col-1 peptide, human; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
| Comments/Corrections | |
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