Document Detail


Jun, Fos and Krox in the thalamus after C-fiber stimulation: coincident-input-dependent expression, expression across somatotopic boundaries, and nucleolar translocation.
MedLine Citation:
PMID:  11744254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expression of the inducible transcription factors Jun, Fos and Krox is commonly used to map neurons in the brain that are activated by sensory inputs. However, some neurons known to be electrically excited by such inputs do not always express these factors. In particular, stimulation of hindlimb sensory nerve C-fibers induces expression of c-Fos in the medial thalamus (the mediodorsal, intermediodorsal, centrolateral and centromedial), but not in the lateral thalamus (the ventroposterolateral, ventroposteromedial and posterior group). We hypothesized that c-Fos expression might only occur in these lateral areas after more complex stimulation patterns, or that only other transcription factors can be induced in these areas by such stimuli. Thus we examined the effects of single, repeated and coincident C-fiber inputs on expression of six inducible transcription factors in the medial, lateral and reticular thalamus of the rat. A weak C-fiber input caused by noxious mechanical stimulation of the skin of one hindpaw did not induce expression of c-Fos, FosB, Krox-20 or Krox-24; but it did reduce the basal expressions of c-Jun and JunD in both the medial and lateral areas. An intense input produced by electrical stimulation of all the C-fibers in one sciatic nerve also failed to induce expression of c-Fos, FosB, Krox-20 or Krox-24 in the medial or lateral areas. However, in the medial thalamus it increased c-Jun and reduced the basal expression of JunD, whereas in the lateral thalamus it had no effect on c-Jun but again reduced the basal expression of JunD. With repeated stimulation, i.e. when the noxious stimulus was applied to the contralateral hindpaw 6 h after the sciatic stimulation, there was again no induction of c-Fos, FosB or Krox-20 in the medial thalamus; but there was an increase in c-Jun and Krox-24, and a decrease in JunD levels. In the lateral thalamus the repeated stimulation again failed to induce c-Fos, but the expressions of FosB, c-Jun and Krox-24 were increased, and that of JunD was again reduced. With coincident stimulation, i.e. when a stimulus was applied to each hindpaw simultaneously, c-Fos and Krox-24 remained absent; but there was a marked induction of FosB and Krox-20, a strong repression of c-Jun, and no effect or a reduction of the basal levels of JunD. This coincident stimulation also caused FosB to appear in the nucleolus of many thalamic neurons. MK-801, but not L-NAME, blocked all these changes. In summary, noxious stimulation affects the expression of all transcription factors in the medial, lateral and reticular thalamus in a complex manner depending upon the inducible transcription factor considered, the thalamic nucleus, and the stimulation paradigm. The expression of some transcription factors uniquely after simultaneous inputs suggests they act as coincidence detectors at the gene level.
Authors:
D D Pearse; G Bushell; J D Leah
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuroscience     Volume:  107     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  2001  
Date Detail:
Created Date:  2001-12-17     Completed Date:  2002-02-01     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  143-59     Citation Subset:  IM    
Affiliation:
School of Biomolecular and Biomedical Sciences, Griffith University, 4111, Nathan, Australia.
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MeSH Terms
Descriptor/Qualifier:
Afferent Pathways / metabolism*
Animals
Bacterial Proteins / metabolism
Cell Nucleolus / metabolism,  ultrastructure
DNA-Binding Proteins / metabolism
Early Growth Response Protein 1
Early Growth Response Protein 2
Electric Stimulation
Enzyme Inhibitors / pharmacology
Excitatory Amino Acid Antagonists / pharmacology
Halothane / pharmacology
Immediate-Early Proteins*
Immunohistochemistry
Male
Nerve Fibers / metabolism*
Nitric Oxide / antagonists & inhibitors,  metabolism
Nociceptors / metabolism*
Pain / genetics,  metabolism*,  physiopathology
Physical Stimulation
Proto-Oncogene Proteins c-fos / metabolism
Proto-Oncogene Proteins c-jun / metabolism
Rats
Rats, Wistar
Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors,  metabolism
Synaptic Transmission / genetics*
Thalamus / cytology,  metabolism*
Transcription Factors / metabolism*
Translocation, Genetic / physiology
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Early Growth Response Protein 1; 0/Early Growth Response Protein 2; 0/Egr1 protein, rat; 0/Egr2 protein, rat; 0/Enzyme Inhibitors; 0/Excitatory Amino Acid Antagonists; 0/Fosb protein, rat; 0/Immediate-Early Proteins; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/Receptors, N-Methyl-D-Aspartate; 0/Transcription Factors; 10102-43-9/Nitric Oxide; 151-67-7/Halothane

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