Document Detail


Joint statistical analysis of multichannel time series from single quantum dot-(Cy5)n constructs.
MedLine Citation:
PMID:  18095665     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One of the major challenges in single-molecule studies is how to extract reliable information from the inevitably noisy data. Here, we demonstrate the unique capabilities of multichannel joint statistical analysis of multispectral time series using Föster resonance energy transfer (FRET) in single quantum dot (QD)-organic dye hybrids as a model system. The multispectral photon-by-photon registration allows model-free determination of intensity change points of the donor and acceptor channels independently. The subsequent joint analysis of these change points gives high-confidence assignments of acceptor photobleaching events despite the interference from background noise and from intermittent blinking of the QD donors and acceptors themselves. Finally, the excited-state lifetimes of donors and acceptors are calculated using the joint maximum likelihood estimation (MLE) method on the donor and acceptor decay profiles, guided by a four-state kinetics model.
Authors:
C Shan Xu; Hahkjoon Kim; Carl C Hayden; Haw Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-12-21
Journal Detail:
Title:  The journal of physical chemistry. B     Volume:  112     ISSN:  1520-6106     ISO Abbreviation:  J Phys Chem B     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-09     Completed Date:  2008-06-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101157530     Medline TA:  J Phys Chem B     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5917-23     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of California at Berkeley, California 94720, USA.
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MeSH Terms
Descriptor/Qualifier:
Carbocyanines / chemistry*
Kinetics
Models, Chemical
Quantum Dots*
Spectrometry, Fluorescence
Time Factors
Chemical
Reg. No./Substance:
0/Carbocyanines; 0/cyanine dye 5

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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