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Jasmonic acid is involved in the signaling pathway for fungal endophyte-induced volatile oil accumulation of Atractylodes lancea plantlets.
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PMID:  22856333     Owner:  NLM     Status:  Publisher    
ABSTRACT: BACKGROUND: Jasmonic acid (JA) is a well-characterized signaling molecule in plant defense responses.However, its relationships with other signal molecules in secondary metabolite productioninduced by endophytic fungus are largely unknown. Atractylodes lancea (Asteraceae) is atraditional Chinese medicinal plant that produces antimicrobial volatiles oils. We incubatedplantlets of A. lancea with the fungus Gilmaniella sp. AL12. to research how JA interactedwith other signal molecules in volatile oil production. RESULTS: Fungal inoculation increased JA generation and volatile oil accumulation. To investigatewhether JA is required for volatile oil production, plantlets were treated with JA inhibitorsibuprofen (IBU) and nordihydroguaiaretic acid. The inhibitors suppressed both JA andvolatile oil production, but fungal inoculation could still induce volatile oils. Plantlets werefurther treated with the nitric oxide (NO)-specific scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt (cPTIO), the H2O2 inhibitorsdiphenylene iodonium (DPI) and catalase (CAT), and the salicylic acid (SA) biosynthesisinhibitors paclobutrazol and 2-aminoindan-2-phosphonic acid. With fungal inoculation, IBUdid not inhibit NO production, and JA generation was significantly suppressed by cPTIO,showing that JA may act as a downstream signal of the NO pathway. Exogenous H2O2 couldreverse the inhibitory effects of cPTIO on JA generation, indicating that NO mediates JAinduction by the fungus through H2O2-dependent pathways. With fungal inoculation, theH2O2 scavenger DPI/CAT could inhibit JA generation, but IBU could not inhibit H2O2production, implying that H2O2 directly mediated JA generation. Finally, JA generation wasenhanced when SA production was suppressed, and vice versa. CONCLUSIONS: Jasmonic acid acts as a downstream signaling molecule in NO- and H2O2-mediated volatileoil accumulation induced by endophytic fungus and has a complementary interaction with theSA signaling pathway.
Cheng-Gang Ren; Chuan-Chao Dai
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Type:  JOURNAL ARTICLE     Date:  2012-8-2
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Title:  BMC plant biology     Volume:  12     ISSN:  1471-2229     ISO Abbreviation:  BMC Plant Biol.     Publication Date:  2012 Aug 
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Created Date:  2012-8-3     Completed Date:  -     Revised Date:  -    
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Nlm Unique ID:  100967807     Medline TA:  BMC Plant Biol     Country:  -    
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Journal ID (nlm-ta): BMC Plant Biol
Journal ID (iso-abbrev): BMC Plant Biol
ISSN: 1471-2229
Publisher: BioMed Central
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Copyright ©2012 Ren and Dai; licensee BioMed Central Ltd.
Received Day: 27 Month: 6 Year: 2012
Accepted Day: 25 Month: 7 Year: 2012
collection publication date: Year: 2012
Electronic publication date: Day: 2 Month: 8 Year: 2012
Volume: 12First Page: 128 Last Page: 128
PubMed Id: 22856333
ID: 3681289
Publisher Id: 1471-2229-12-128
DOI: 10.1186/1471-2229-12-128

Jasmonic acid is involved in the signaling pathway for fungal endophyte-induced volatile oil accumulation of Atractylodes lancea plantlets
Cheng-Gang Ren1 Email:
Chuan-Chao Dai1 Email:
1Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Science, Nanjing Normal University, Nanjing, 210046, P.R. China


Atractylodes lancea, a member of the Compositae family, is a traditional Chinese medicinal plant [1,2]. Volatile oils from A. lancea show antimicrobial activities as well. These oils comprise active secondary metabolites, including the characteristic components atractylone, β-eudesmol, hinesol, and atractylodin [3]. Secondary metabolites, such as terpenes, flavonoids, and alkaloids, are believed to be involved in plant responses to many biotic and abiotic stresses [4-6]. Another plant defense response is the activation of multiple signaling events [7,8]. For example, jasmonic acid (JA) biosynthesis by plants is induced by pathogen infection and elicitor treatment [9], and salicylic acid (SA) is involved in activating distinct sets of defense-related genes [10], such as those that encode pathogenesis-related (PR) proteins [11]. Also, many signaling molecules have been revealed to be involved in secondary metabolism [12-14].

Endophytes can coexist with their hosts and have great potential to affect the hosts’ metabolism [15]; their effects on plant accumulation of medicinal components have received much attention recently [16,17]. Unlike pathogens, endophytic fungi do not cause strong hypersensitive reactions in the host. But long-term colonization can induce various kinds of metabolites to accrue in hosts [17,18]. How endophytic fungus-host interactions affect the accumulation of plant secondary metabolites is an intriguing issue.

Jasmonic acid is a well-characterized plant signaling molecule that mediates plant defense responses [19] by responding to microbial infection and elicitor treatment [20]. Kunkel et al..[21] found that fungal elicitor caused rapid increases in JA production, secondary metabolite biosynthetic gene expression, and secondary metabolite accumulation in many plants. Exogenous JA application enhanced gene expression of secondary metabolite biosynthetic pathways, while the fungal elicitor-induced secondary metabolite accumulation could be abolished by JA synthesis inhibitors [13]. Most plant defense responses are regulated by many signal molecules, and “cross-talk” among multiple signaling pathways is important in plant cell signal transduction networks [21]. An increasing number of studies have shown that these signals do not function entirely independently; rather, they are influenced the magnitude or amplitude of various other signals [22].

Although interactions between SA- and JA-mediated signaling pathways have been reported to enhance the expression of plant defense-related genes, studies on interactions between JA and multiple signaling pathways (nitric oxide, hydrogen peroxide, and SA) in mediating plant secondary metabolite accumulation are rare. In this work, we report that JA acts as a downstream signal of nitric oxide (NO)- and hydrogen peroxide (H2O2)-mediated volatile oil accumulation in A. lancea plantlets induced by endophytic fungus Gilmaniella sp. AL12. Furthermore, we reveal an unusual complementary relationship between JA and SA in mediating the biosynthesis of medicinal plant secondary metabolites.

Plant materials and treatments

Meristem cultures of Atractylodes lancea (collected in Maoshan, Jiangsu Province, China) were established according to Wang et al. [22]. The explants were surface sterilized and grown in MS medium [23] supplemented with 0.3 mg/L naphthaleneacetic acid (NAA), 2.0 mg/L 6-benzyladenine, 30 g/L sucrose, and 10% agar in 150 mL Erlenmeyer flasks. Rooting medium (1/2 MS) contained 0.25 mg/L NAA, 30 g/L sucrose, and 10% agar. All media were adjusted to a pH of 6.0 before being autoclaved. Cultures were maintained in a growth chamber (25/18°C day/night, with a light intensity of 3400 lm/m2 and a photoperiod of 12 h) and subcultured every four weeks. Thirty-day-old rooting plantlets were used for all treatments.

Reagents used as specific scavengers or inhibitors, including ibuprofen (IBU), nordihydroguaiaretic acid (NDGA), 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline −1-oxyl-3-oxide potassium salt (cPTIO), paclobutrazol (PAC), catalase (CAT), diphenylene iodonium (DPI), and 2-aminoindan-2-phosphonic acid (AIP), were purchased from Sigma-Aldrich (St. Louis, MO, USA). All exogenous signaling molecules and inhibitors were filtered using 0.22 μm diameter microporous membranes before use. Unless stated otherwise, inhibitors were applied 1 d before the application of signaling molecules or fungal inoculation.

Fungal culture and treatments

The endophytic fungus AL12 (Gilmaniella sp.) was isolated from A. lancea, cultured on potato dextrose agar, and incubated at 28°C for five days [24]. Thirty-day-old plantlets were inoculated using 5-mm AL12 mycelial disks. An equal size of potato dextrose agar was used as a control. All treatments were conducted in a sterile environment and replicated at least three times to examine reproducibility.

Measurement of H2O2 and NO

Thirty-day-old plants were incubated with fungal mycelia disks with or without inhibitors and were harvested 18 d later for determination of NO or H2O2. Inhibitors were 1.25 mmol L-1 cPTIO, 5.25 mKat L-1 CAT or 3 mmol L-1 DPI.

The generation of H2O2 by A. lancea plantlets was measured by chemiluminescence in a ferricyanide-catalyzed oxidation of luminol according to Schwacke and Hager [25], with modification. Leaf samples (1 g) were ground with 5 ml double distilled water. The homogenate was centrifuged at 13,000 g for 10 min, then 100 μL supernatant, 50 μL luminol (5-amino-2,3-dihydro-l,4-phthalazinedione), and 800 μL phosphate-buffered saline were mixed in a cuvette. The reaction was initiated with 100 μL K3[Fe(CN)6. To compare independent experiments, we used H2O2 as an internal standard. Fifty microliters of H2O2 (1 μM, freshly prepared) was added to the assay mixture containing 750 μL potassium phosphate buffer. One unit of H2O2 was defined as the chemiluminescence caused by the internal standard of 1 μM H2O2 per gram fresh weight.

The generation of NO was monitored using a NO detection kit (Nanjing Jiancheng Bio-engineering Inst., Nanjing, China) according to the manufacturer’s instructions. Leaf samples (1 g) were ground with 5 ml of 40 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (pH 7.2) and the homogenate was centrifuged at 14,000 g for 10 min. The supernatant was used for the NO assays. One unit of NO was defined as the absorbance variation caused by the internal standard of 1 μM NO per gram fresh weight.

At least 15 plantlets were assayed for each time point, and all treatments were performed in triplicate.

Measurement of SA

Thirty-day-old plants were incubated with fungal mycelia disks with or without inhibitors and were harvested 18 d later for determination of SA. Inhibitors were 1 mmol L-1 PAC or 2.5 mmol L-1 AIP.

Salicylic acid was extracted followed the method of Verberne et al. [26], with some modifications. Five grams of whole plantlets was ground in liquid nitrogen and extracted in 2 ml methanol by sonication. After centrifugation at 14,000 g for 5 min, the supernatant was rotary evaporated, and the residue was resuspended in 250 μl of 5% trichloroacetic acid. The mixture was re-extracted with 800 μl acetic acid ester: cyclohexane (1:1 v/v). Finally, the organic phase was rotary evaporated until dry, dissolved with 600 μl HPLC mobile phase (methanol: 2% acetic acid: H2O, 50:40:10, v: v: v), and filtered with a 0.22-μm microporous membrane for determination.

The SA samples were quantified by HPLC using a reverse-phase column (Hedera Packing Material Lichrospher 5-C18, 4.6 × 250 mm, 5 μm, Bonna-Agela Technologies, Wilmington, DE, USA). The mobile phases flow rate was 1 ml min−1. Salicylic acid was detected at 217 nm at 25°C [14].

Extraction and determination of volatile oils and JA

Thirty-day-old plantlets of Atractylodes lancea were incubated with 5-mm mycelial disks or PDA disks (control). Inhibitors (0.1 mmol L-1 IBU or NDGA) were added 1 d before fungal inoculation for JA determination.

Volatile oils were extracted from whole plantlets of A. lancea, including leaves and rhizomes (0.8–1.6% oil content in leaves, 2.2–3.4% in rhizomes), according to Zhang et al. [27]. The volatile oils were dried with anhydrous sodium sulfate and stored in dark glass bottles at 4°C for gas chromatograph (GC) analysis.

Following Juergen et al. [28], JA was extracted by grinding plant material (1 g) frozen in liquid nitrogen and extracting with H2O: acetone (30:70, v:v). Samples were store in dark glass bottles at −21°C for GC analysis.

GC determination was carried out using an 1890 series GC (Hewlett-Packard, Palo Alto, CA) equipped with a flame ionization detector. A DB-5 ms (30 m × 0.25 mm × 0.25 μm) column (Agilent, Santa Clara, CA, USA) was used with the following temperature program: column held at 60°C for 1 min after injection, increased by 10°C/min to 190°C, held for 2 min, increased by 5°C/min to 210°C, held for 2 min, increased by 10°C/min to 220°C, and held for 8 min. Nitrogen was used as carrier and the flow rate was 4 ml/min. Four main components of the volatile oils, atractylone, hinesol, β-eudesmol, and atractylodin, were quantitatively analyzed according to the method of Fang et al. [29]; their retention times were 14.57, 15.24, 16.21, and 22.18 min, respectively.

Real-time quantitative RT-PCR analysis

Total RNA was extracted from leaves as described by Dong and Beer [30]. First-strand cDNA was synthesized from 1 μg of total RNA (PrimeScript RT Reagent Kit, Takara, Dalian, China). Real-time qPCR was performed using the DNA Engine Opticon 2 Real-time PCR Detection System (Bio-Rad, Hercules, CA, USA) and SYBR green probe (SYBR Premix Ex Taq system, Takara). The constitutively-expressed gene EF1α used as an internal positive control. The gene-specific primers used to amplify EF1α were 5′-CAGGCTGATTGTGCTGTTCTTA-3′ and 5′-TGTGGCATCCATCTTGT-3′ (241 bp product) and for alHMGR were 5′-GGTGAGAAAGGTCCTGAAA-3′ and 5′-CATGGTAACGGAGATATGAA-3′ (154 bp). The GenBank accession numbers of the alHMGR and EF1α genes are EF090602.1 and X97131, respectively.

The thermocycler program was as follows: 90 s at 95°C; 40 cycles of 30 s at 95°C, 30 s at 57°C, and 30 s at 72°C; and 5 min at 72°C. To standardize the data, the ratio of the absolute transcript level of the alHMGR genes to the absolute transcript level of EF1α was calculated for each sample of each treatment.

Statistical analyses

Data were compiled using Microsoft Excel (Redmond, WA, USA). The values were represented as mean ± SD of three replicates for each treatment. Student’s t-test, one-way ANOVA, and Duncan’s multiple range test were used to identify significant differences (SPSS ver. 13.0, SPSS Inc., Chicago, IL, USA).

Dependence of JA in fungus-induced volatile oil accumulation

The JA contents of the plantlets increased significantly after endophytic fungus inoculation (Figure 1A), indicating that the fungus may trigger JA biosynthesis in the plantlets. Concurrently, the total amount of volatile oils increased significantly (Table 1). Both IBU and NDGA are inhibitors of the octadecanoid pathway that synthesizes JA and are usually applied in plant systems as JA-specific inhibitors [13]. To investigate whether JA was involved in the fungus-induced volatile oil accumulation, IBU and NDGA were applied; as shown in Figure 1B, both inhibitors suppressed not only the fungus-induced JA generation, but also the fungus-triggered volatile oil production. The results suggested that JA was important for fungus-induced volatile-oil synthesis in A. lancea plantlets. However, volatile oils in the A. lancea plantlets treated with both fungus and JA inhibitors could still accumulate, compared with the control, even though JA generation was lower than control (Figure 1B), implying that fungus-induced volatile oil synthesis is not solely dependent on the JA signaling pathway.

JA acts as a downstream signal of NO and H2O2 pathway

Previous results showed that JA is not the sole signaling pathway involved in fungus-induced volatile oil synthesis; NO, H2O2, and SA are also known to mediate this process in A. lancea plantlets [22]. To investigate a possible relationships between JA and one or more of these other pathways, A. lancea plantlets were treated with the NO-specific scavenger cPTIO, the membrane NADPH oxidase inhibitor DPI/CAT, the SA inhibitor PAC/AIP, IBU, and fungal inoculation. The NO scavenger cPTIO could inhibit JA production in inoculated plantlets, but IBU could not inhibit NO production (Figure 2A), showing that JA may act as a downstream signal of NO. Exogenous H2O2 could reverse JA suppression, implying that JA is mediated by NO though H2O2 in endophyte-induced volatile-oil accumulation. In addition, the H2O2 inhibitor DPI/CAT could inhibit JA production, but IBU could not inhibit H2O2 production with inoculation (Figure 2A). The one-way dependence of JA on H2O2 confirmed that H2O2 was the intermediary factor between JA and NO.

Paclobutrazol is an effective SA biosynthesis-related benzoic acid hydroxylase (BA2H) inhibitor [31] that also inhibits gibberellin biosynthesis [32]. Therefore, we also used AIP, a specific SA biosynthesis-related phenylalanine ammonialyase (PAL) inhibitor [33,34], to confirm that SA generation was suppressed. Interestingly, PAC and AIP could abolish the suppression of JA by DPI/CAT with fungus inoculation (Figure 2B). This result implied that the SA and JA signaling pathways were closely linked in endophyte-induced volatile-oil accumulation in A. lancea plantlets.

Complementary interactions between JA and SA in fungus-induced volatile-oil accumulation

To further investigate the relationship between JA and SA, gradient concentrations of the JA-inhibiter IBU and the SA-inhibitors PAC and AIP were applied. As shown in Figure 3, the fungus-induced JA level of the plantlets decreased gradually as IBU concentration increased, but both SA accumulation and volatile oil content were enhanced as well, although the amounts did not exceed those obtained with fungal inoculation alone. Similarly, SA levels in plantlets were inhibited by 0.1, 1, and 2.5 mmol L-1 AIP and by 3 mmol L-1 PAC, whereas JA was enhanced significantly (Figure 3A). Volatile oil accumulation was enhanced by 2.5 mmol L-1 AIP and 3 mmol L-1 PAC (Figure 3B). The results suggested that JA may have a complementary interaction with SA to mediate fungal endophyte-induced volatile-oil accumulation. However, combining IBU and paclobutrazol could not completely inhibit volatile oil synthesis. We added the H2O2-inhibitor DPI/CAT to IBU and paclobutrazol, which reduced volatile-oil accumulation to the level of the control. The results suggested that H2O2, SA, and JA may work simultaneously in fungus-induced volatile-oil synthesis in A. lancea plantlets.

Dependence of fungus-induced sesquiterpenoid production on JA production

The enzyme 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) catalyzes the conversion of HMG-CoA to mevalonate, which is the key step in the terpenoid biosynthesis pathway in plants [35,36]. We further investigated the possible mediating role of JA on HMGR gene expression. The results showed that exogenous JA could strongly stimulate HMGR gene expression (Figure 4A). Three sesquiterpenoid components of A. lancea volatile oils, atractylone, β-eudesmol, and hinesol, were all induced by JA and suppressed by IBU with fungal inoculation (Figure 4B).


Secondary metabolite accumulation is a common plant response to biotic or abiotic environmental stress, and secondary messengers are widely employed to mediate the accumulation of plant secondary metabolites. This work demonstrated that the fungus Gilmaniella sp. can induce JA production and promote the accumulation of volatile oils in host plantlets. As an important signal molecule, JA plays key roles in regulating the induction of volatile oils by the endophytic fungus. The specific inhibitors IBU and NDGA could block the JA signaling pathway and reduce the accumulation of related metabolites. Our previous study showed that NO, H2O2, and SA acted as signal molecules to mediate the accumulation of volatile oils in suspension cells of A. lancea caused by endophytic fungal elicitor [22]. Thus, the possible relationships between JA and other known signaling pathways in the accumulation of secondary metabolites were further investigated.

Cross-talk between different signal transduction pathways, as opposed to single signaling pathways, mediates gene expression and the production of secondary metabolites during plant defense responses [37,38]. Hydrogen peroxide has been reported to be a possible upstream signal for NO production in mung bean plantlets [39]. Nitric oxide also can mediated fungal elicitor-induced taxol biosynthesis in Taxus chinensis suspension cells through reactive oxygen signaling pathways, stimulate SA accumulation in tobacco cell cultures, and induce PAL expression via an SA independent pathway [31,40,41]. Moreover, our previous work demonstrated that NO mediates volatile oil accumulation induced by the fungus through SA- and H2O2-dependent pathways. Hydrogen peroxide can enhance SA production but does not act as upstream signal molecule [22]. The present work showed that endophytic fungus-induced JA was directly mediated by H2O2 and acted as a downstream signal molecule for both H2O2 and NO pathways.

In our study, JA had an unusual complementary interaction with the SA signaling pathway. Jasmonic acid is commonly postulated to act antagonistically on the SA signaling pathway and on the expression of SA-dependent genes [42,43]. Other studies have shown that SA is a potent suppressor of JA signaling pathways and JA-dependent defense gene expression in various pharmacological and genetic experiments [44,45]. In addition, both JA and SA are important signaling molecules in plant defense responses, such as the activation of distinct sets of defense-related genes and the development of systemic acquired resistance [21,46]. Our results showed that when JA biosynthesis was suppressed by the inhibitor IBU, accumulation of SA was enhanced to compensate for the loss of JA-mediating function in fungus-triggered volatile-oil production. Similarly, JA production/signaling could substitute for the SA pathway when SA accumulation was impaired.


The value of medicinal herbs relies mainly on the accumulation of active pharmaceutical ingredients; low yield is the main challenge to producing high-quality herbs. In this work, we demonstrated that JA acts as a downstream signaling molecule in NO- and H2O2-mediated volatile oil accumulation induced by endophytic fungus and has a complementary interaction with the SA signaling pathway and clarified that HMGR gene expression was significantly stimulated by JA along with increasing sesquiterpenoid components. This information will help to better understand the relationships between fungal endophytes and their host plants. Furthermore, it also suggests strategies to improve the quality of medicinal herbs.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

CGR designed experiment, analyzed data, and wrote the manuscript. CCD supervised the work and interpreted data with CGR. Both authors read and approved the final manuscript.


The authors are grateful to the National Natural Science Foundation of China (grant nos. 31070443 and 30500066) and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions for financial support.

Duan JA,Wang LY,Qian SH,Su SL,Tang YP,A new cytotoxic prenylated dihydrobenzofuran derivative and other chemical constituents from the rhizomes of Atractylodes lancea DCArch Pharm ResYear: 200812896596910.1007/s12272-001-1252-z18787781
Yuan Y,Liu YJ,Huang LQ,Cui GH,Fu GF,Soil acidity elevates some phytohormone and β-Eudesmol contents in Roots of Atractylodes lanceaRuss J Plant PhysiolYear: 200912113313710.1134/S1021443709010191
Wang Y,Dai CC,Chen Y,Antimicrobial activity of volatile oil from Atractylodes lancea against three species of endophytic fungi and seven species of exogenous fungiChinese J Appl EcolYear: 2009121127782784 in Chinese.
Rodriguez RJ,White JJF,Arnold AE,Redman RS,Fungal endophytes: diversity and functional rolesNew PhytolYear: 200912231433010.1111/j.1469-8137.2009.02773.x19236579
Mucciarelli M,Camusso W,Maffei M,Panicco P,Bicchi C,Volatile terpenoids of endophyte-free and infected peppermint (Mentha piperita L.): chemical partitioning of a symbiosisMicrob EcolYear: 200712468569610.1007/s00248-007-9227-017370029
Wang AM,Zhang FK,Huang LF,Yin XP,Li HF,Wang QY,et al. New progress in biocatalysis and biotransformation of flavonoidsJ Med Plants ResYear: 20101210847856
John MM,Jeffery LD,Signal transduction in the plant immune responseTrends in biochemical scienceYear: 2000122798210.1016/S0968-0004(99)01532-7
Hahlbrock K,Bednarek P,Ciolkowski I,Hamberger B,Heise A,Liedgens H,Logemann E,Nurnberger T,Schmelzer E,Somssich IE,Tan JW,Non-self recognition, transcriptional reprogramming, and secondary metabolite accumulation during plant/pathogen interactionsProc Natl Acad Sci USAYear: 200312Suppl 2145691457612704242
Ward ER,Uknes SJ,Williams SC,Dincher SS,Wiederhold DL,Alexander DC,Ahl-Goy P,Métraux JP,Ryals JA,Coordinate gene activity in response to agents that induce systemic acquired resistancePlant CellYear: 199112101085109412324583
Kazan K,Manners JM,Jasmonate signaling: toward an integrated viewPlant PhysiolYear: 20081241459146810.1104/pp.107.11571718390489
Van LC,Van EA,The families of pathogenesis related proteins, their activities, and comparative analysis of PR-1 type proteinsPhysiolYear: 1999122859710.1006/pmpp.1999.0213
Yuan Y,Li C,Hu Z,Signal transduction pathway for oxidative burst and taxol production in suspension cultures of Taxus chinensis vat mairei induced by oligosaccharide from Fusarium oxysprumEnzyme Microb TechnolYear: 200112737237910.1016/S0141-0229(01)00406-9
Nojiri H,Sugimori M,Yamane H,Nishimura Y,Yamada A,Shibuya N,Kodama O,Murofushi N,Omori T,Involvement of jasmonic acid in elicitor-induced phytoalexin production in suspension-cultured rice cellsPlant PhysiolYear: 199612238739212226190
Gao FK,Ren CG,Dai CC,Signaling Effects of Nitric Oxide, Salicylic Acid, and Reactive Oxygen Species on Isoeuphpekinensin Accumulation in Euphorbia pekinensis Suspension Cells Induced by an Endophytic Fungal ElicitorJ Plant Growth RegulYear: 201210.1007/s00344-012-9258-8
Suryanarayanana TS,Thirunavukkarasub N,Govindarajulub MB,Sassec F,Jansend R,Muralia TS,Fungal endophytes and bioprospectingFungal Biology ReviewsYear: 200912291910.1016/j.fbr.2009.07.001
Wang LW,Xu BG,Wang JY,Su ZZ,Lin FC,Zhang CL,Kubicek CP,Bioactive metabolites from Phoma species, an endophytic fungus from the Chinese medicinal plant Arisaema erubescensApplied Microbial and Cell PhysiologyYear: 201212312311239
Saunders M,Kohn LM,Evidence for alteration of fungal endophyte community assembly by host defense compoundsNew PhytolYear: 200912122923810.1111/j.1469-8137.2008.02746.x19170900
Li YC,Tao WY,Interactions of taxol-producing endophytic fungus with its host (Taxus spp.) during taxol accumulationCell Biol IntYear: 200912110611210.1016/j.cellbi.2008.10.00718996212
Halim VA,Vess A,Scheel D,Rosahl S,The Role of Salicylic Acid and Jasmonic Acid in Pathogen DefencePlant BiolYear: 200612230731316807822
Zhao J,Davis LC,Verpoorte R,Elicitor signal transduction leading to production of plant secondary metabolitesBiotechnol AdvYear: 200512428333310.1016/j.biotechadv.2005.01.00315848039
Kunkel BN,Brooks DM,Cross talk between signaling pathways in pathogen defenseCurr Opin Plant BiolYear: 200212432533110.1016/S1369-5266(02)00275-312179966
Wang Y,Dai CC,Zhao YW,Peng Y,Fungal endophyte-induced volatile oil accumulation in Atractylodes lancea plantlets is mediated by nitric oxide, salicylic acid and hydrogen peroxideProcess BiochemYear: 201112373073510.1016/j.procbio.2010.11.020
Murashige T,Skoog F,A revised medium for rapid growth and bioassays with tobacco tissue culturePhysiol PlantYear: 196212347347910.1111/j.1399-3054.1962.tb08052.x
Chen JX,Dai CC,Li X,Tian LS,Xie H,Endophytic fungi screening from Atracty lancea and inoculating into the host plantletGuihaiaYear: 2008122256260
Schwacke R,Hager A,Fungal elicitors induce a transient release of active oxygen species from cultured spruce cells that is dependent on Ca2+and protein-kinase activityPlantaYear: 1992121136141
Verberne MC,Brouwer N,Delbianco F,Linthorst HJM,Bol HF,Verpoorte R,Method for the extraction of the volatile compound salicylic acid from tobacco leaf materialPhytochem AnalYear: 2002121455010.1002/pca.61511899606
Zhang B,Dai CC,Fang F,Chen JX,Effects of three species endophytic fungi on Atractylodes lancea growth and its essential oil compositionChin J EcolYear: 200912704709 in Chinese.
Juergen E,Eric AS,Hans TA,Yasmin JC,Juan H,James HT,Simultaneous quantification of jasmonic acid and salicylic acid in plants by vapor-phase extraction and gas chromatography-chemical ionization-mass spectrometryAnal BiochemYear: 200312224225010.1016/S0003-2697(02)00466-912531212
Fang F,Dai CC,Zhang B,Liang QL,Establishment of suspension cell line of Atractylodes lancea and effect of endophytic fungal elicitors on its essential oil accumulationChin Tradit Herbal DrugsYear: 2009123452455 in Chinese.
Dong H,Beer SV,Riboflavin induces disease resistance in plants by activating a novel signal transduction pathwayPhytopathologyYear: 200012880181110.1094/PHYTO.2000.90.8.80118944500
Leon J,Lawton MA,Raskin L,Hydrogen peroxide stimulates salicylic acid biosynthesis in tobaccoPlant PhysiolYear: 199512416371678
Shiow YW,Tung S,Miklos F,Translocation of Paclobutrazol, a Gibberellin Biosynthesis Inhibitor, in Apple SeedlingsPlant PhysiolYear: 1986121111410.1104/pp.82.1.1116664976
Zon J,Amrhein N,Inhibitors of phenylalanine ammonia-lyase:2-aminoindan-2- phosphonic acid and related compoundsEur J Org ChemYear: 199212625628
Willibald S,Naoko K,Dieter S,The decisive step in betaxanthin biosynthesis is a spontaneous reactionPlant PhysiolYear: 19991241217123210.1104/pp.119.4.121710198080
Chappell J,Wolf F,Proulx J,Cuellar R,Saunders C,Is the reaction catalyzed by 3-hydroxyl-methylglutaryl coenzyme A reductase a rate-limiting step for isoprenoid biosynthesis in plants?Plant PhysiolYear: 19951241337134312228673
Ha SH,Kim JB,Hwang YS,Lee SW,Molecular characterization of three 3-hydroxy-3-methylglutaryl -CoA reductase genes including pathogen-induced Hmg2 from pepper (Capsicum annuum)Biochimica et Biophys. ActaYear: 200312325326010.1016/S0167-4781(02)00624-3
Reymond P,Farmer EE,Jasmonate and salicylate as global signals for defense gene expressionCurr Opin Plant BiolYear: 199812540441110.1016/S1369-5266(98)80264-110066616
Mur LAJ,Kenton P,Atzorn R,Miersch O,Wasternack C,The outcomes of concentration-specific interactions between salicylate and jasmonate signaling include synergy, antagonism, and oxidative stress leading to cell deathPlant PhysiolYear: 200612124926216377744
Lum HK,Butt YKC,Lo SCL,Hydrogen peroxide induces a rapid production of nitric oxide in Mung bean (Phaseolus aureus)Nitric Oxide-Biol ChYear: 200212220521310.1006/niox.2001.0395
Chamnongpol S,Willekens H,Moeder W,Langebartels C,Sandermann H,Van MM,Inzé D,Van CW,Defense activation and enhanced pathogen tolerance induced by H2O2 in transgenic tobaccoProc Natl Acad Sci USAYear: 199812105818582310.1073/pnas.95.10.58189576968
Xu MJ,Dong JF,Zhu MY,Nitric oxide mediates the fungal elicitor-induced Taxol biosynthesis of Taxus chinensis suspension cells through the reactive oxygen species-dependent and -independent signal pathwaysChin Sci BullYear: 200612219671975
Kachroo P,Shanklin J,Shah J,Whittle EJ,Klessig DF,A fatty acid desatuase modulates the activation of defense signaling pathways in plantsProc Natl Acad Sci USAYear: 200112169448945310.1073/pnas.15125839811481500
Kloek AP,Verbsky ML,Sharma SB,Schoelz JE,Vogel J,Klessig DF,Kunkel BN,Resistance to Pseudomonas syringae conferred by an Arabidopsis thaliana coronatineinsensitive (coil) mutation occurs through two distinct mechanismsPlant JYear: 200112550952210.1046/j.1365-313x.2001.01050.x11439137
Steven HS,Annemart K,Susanne M,Claessens C,Jerôme PK,Johan AVP,Martin JM,Antony JB,Jean-Pierre M,Rebecca B,Kemal K,Van Loon LC,Xinnian D,Corné MJP,NRP1 modulates cross-talk between salicylate- and jasmonate dependent defense pathways through a novel function in the cytosolPlant CellYear: 200312360770
Li J,Brader G,Palva ET,The WRKY70 transcription factor: a node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defensePlant CellYear: 200412231933110.1105/tpc.01698014742872
Shah J,The salicylic acid loop in plant defenseCurr Opin Plant BiolYear: 200312436537110.1016/S1369-5266(03)00058-X12873532


[Figure ID: F1]
Figure 1 

Endophytic fungus-induced volatile-oil accumulation is dependent on JA generation. Thirty-day-old plantlets of Atractylodes lancea were incubated with 5-mm mycelial disks or PDA disks (control). (A) Jasmonic acid production at 2-day intervals. Asterisks indicate significant differences from the control (0 d) (t-test; *, P <0.05; **, P <0.01). (B) Effects of JA inhibitors on endophytic fungus-induced volatile-oil accumulation after 18 d. Inhibitors (0.1 mmol L-1 IBU or NDGA) were added 1 d before fungal inoculation. Values are means of three independent experiments. Bars with different lower-case letters were significantly different (one-way ANOVA, Duncan’s multiple range test, P <0.05).

[Figure ID: F2]
Figure 2 

Interactions between JA and NO or H2O2signaling pathways induced by endophytic fungus. Thirty-day-old plantlets of Atractylodes lancea were incubated with 5-mm mycelia disks with or without inhibitors and were harvested 18 d later for determination of JA and NO or H2O2 concentrations. (A) Interactions between JA and NO pathways. Inhibitors were 1.25 mmol L-1 cPTIO, 0.1 mmol L-1 IBU, or 15 mmol L-1 H2O2. (B) Interactions between JA and H2O2. Inhibitors were 3 mmol L-1 DPI, 5.25 mKat L-1 CAT, 0.1 mmol L-1 IBU, 1 mmol L-1 PAC, or 2.5 mmol L-1 AIP. All inhibitors were added 1 d before fungus inoculation. Values are means of three independent experiments. Bars with different lower-case letters were significantly different (one-way ANOVA, Duncan’s multiple range test, P <0.05).

[Figure ID: F3]
Figure 3 

Complementary interaction between JA and SA signaling pathways induced by endophytic fungus. Thirty-day-old plantlets of Atractylodes lancea were incubated with 5-mm mycelia disks and 0.1, 1, or 3 mmol L-1 IBU; 3 mmol L-1 DPI; or 0.1, 1, or 2.5 mmol L-1 AIP and 3 mmol L-1 PAC. Plants were harvested 18 d later to determine JA and volatile oil levels. Inhibitors were added 1 d before fungus inoculation. (A) Interactions between JA and SA pathways. (B) Volatile oil production. Values are means of three independent experiments. Bars with different lower-case letters were significantly different (one-way ANOVA, Duncan’s multiple range test, P <0.05).

[Figure ID: F4]
Figure 4 

Expression levels of HMGR genes and sesquiterpenoid accumulation responses to JA signaling pathway.(A) Expression levels of EF1α and HMGR genes in response to JA determined by real-time qPCR and semi-qPCR analysis. Thirty-day-old plantlets of Atractylodes lancea were incubated with 5-mm mycelia disks; 0.01, 0.1, or 1 mmol L-1 JA; or 1 mmol L-1 IBU and harvested 18 d later for total RNA extraction and PCR analysis. Values are means ± SE (n = 3). Asterisks indicate significant differences (t-test; *, P <0.05; **, P <0.01). (B) Effects of JA on sesquiterpenoid accumulation. Plantlets were harvested after 18 d and evaluated for atractylone, β-eudesmol, and hinesol content. Values are means ± SE (n = 3). Bars with different lower-case letters were significantly different (one-way ANOVA, Duncan’s multiple range test, P <0.05).

[Figure ID: F5]
Figure 5 

Cross-talk between signaling pathways for volatile oil accumulation induced by endophytic fungal elicitor. ‘’ indicates that the signal molecule was suppressed by specific inhibitors, while positive regulation is shown as ‘’.

[TableWrap ID: T1] Table 1 

Accumulation of volatile oils byAtractylodes lanceaover time

Components Treatment 0 day 4 day 6 day 8 day 10 day 12 day 14 day 16 day 18 day 20 day
Atractylone (μg/g)
4.63 ± 1.41a
4.23 ± 0.74a
5.24 ± 0.94a
4.41 ± 0.67a
4.97 ± 0.56a
8.64 ± 1.19b
13.48 ± 1.54c
23.53 ± 2.76d
28.43 ± 1.54d
15.13 ± 0.93c
4.63 ± 1.27a
4.92 ± 1.02a
3.97 ± 0.42a
5.2 ± 0.55a
3.15 ± 0.75a
3.92 ± 0.48a
4.31 ± 0.39
4.71 ± 0.44a
5.17 ± 0.63a
5.6 ± 0.52a
Hinesol (μg/g)
38.17 ± 4.32a
40.12 ± 3.82a
41.6 ± 4.93a
40.85 ± 5.63a
54.42 ± 4.23b
65.15 ± 5.28c
78.72 ± 6.63d
104.42 ± 8.23e
128 ± 9.42f
52.15 ± 4.45b
38.17 ± 5.36a
32.31 ± 3.52a
38.63 ± 3.78a
35.62 ± 3.29a
43.81 ± 4.22a
46.95 ± 3.04a
37.13 ± 6.27a
46.21 ± 3.23a
46.9 ± 3.32a
50.22 ± 5.24a
β-Eudesmol (μg/g)
80.72 ± 11.37a
85.6 ± 6.01a
92.23 ± 6.43a
96.63 ± 6.48b
104.75 ± 6.12c
104.75 ± 6.06c
116.58 ± 6.19d
119.62 ± 6.25e
123.83 ± 8.07e
99.65 ± 4.18c
80.72 ± 10.75a
78.7 ± 8.32a
81.27 ± 8.53a
88.51 ± 7.95a
93.18 ± 8.28a
94.67 ± 8.05a
98.38 ± 5.04a
96.42 ± 8.15a
85.1 ± 8.18a
94.77 ± 7.84a
Atractylodin (μg/g)
98.32 ± 14.53a
109.24 ± 11.31a
111.23 ± 12.95a
118.97 ± 12.74a
125.53 ± 17.85a
131.52 ± 12.34a
137.64 ± 15.31b
152.34 ± 12.92b
171.63 ± 12.04b
183.4 ± 12.39c
98.32 ± 12.75a
110.7 ± 10.61a
114.2 ± 7.76a
115.42 ± 8.23a
121.9 ± 10.28a
111.47 ± 12.71a
116.8 ± 10.07a
118.5 ± 10.63a
121.1 ± 10.75a
134.1 ± 10.68a
Total (μg/g)
221.84 ± 31.63a
239.19 ± 21.88a
250.3 ± 25.25a
260.86 ± 25.52b
289.67 ± 28.76c
310.06 ± 24.87d
346.42 ± 29.67e
399.91 ± 30.15f
451.89 ± 31.07 g
350.33 ± 21.95e
  Control 221.84 ± 30.13a 226.63 ± 23.47a 238.07 ± 20.49a 244.75 ± 19.77a 262.04 ± 23.53a 257.01 ± 24.28a 256.62 ± 21.77a 265.84 ± 22.45a 258.27 ± 22.85a 284.69 ± 24.28a

Thirty-day-old plantlets were incubated with 5-mm mycelia disks or with an equal size of potato dextrose agar (control). Data are mean ± standard deviation (SD) of triplicate samples. Within each row, values followed by different lower-case letters were significantly different (one-way ANOVA, Duncan’s multiple range test, P <0.05).

Article Categories:
  • Research Article

Keywords: Atractylodes lancea, Endophytic fungi, Volatile oil, Jasmonic acid, Medicinal herb.

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