Document Detail


Jacalin: a new laboratory tool in immunochemistry and cellular immunology.
MedLine Citation:
PMID:  2668478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In recent years, a new lectin--jacalin--has raised the interest of immunologists because of its original properties with respect to human immunoglobulins and lymphocytes. Its structure and carbohydrate binding specificity are now well documented, and it can be purified easily from jackfruit seeds by ion exchange or affinity chromatography. The binding and precipitating specificities of jacalin with heavy chains of human immunoglobulins allow its use as a diagnostic (IgA subclass typing) and preparative tool (purification of IgA and IgD, removal of IgA from biologic samples and preparations). Other possible applications of jacalin's binding properties also can be envisaged. In addition, the lectin displays a mitogenic activity specific for human CD4 T-lymphocytes; consequently, the proliferative response induced by jacalin appears to represent a new and interesting assay for a functional study of CD4 cells, with obvious applications in primary and acquired, especially AIDS, immune deficiency states.
Authors:
P Aucouturier; N Pineau; J C Brugier; E Mihaesco; F Duarte; F Skvaril; J L Preud'homme
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Journal of clinical laboratory analysis     Volume:  3     ISSN:  0887-8013     ISO Abbreviation:  J. Clin. Lab. Anal.     Publication Date:  1989  
Date Detail:
Created Date:  1989-09-18     Completed Date:  1989-09-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8801384     Medline TA:  J Clin Lab Anal     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  244-51     Citation Subset:  IM; S    
Affiliation:
Laboratory of Immunology and Immunopathology, CNRS URA 1172, University Hospital, Poitiers, France.
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MeSH Terms
Descriptor/Qualifier:
Carbohydrates
Immunochemistry
Immunoglobulins / isolation & purification
Immunologic Techniques
Lectins* / immunology,  isolation & purification,  pharmacology
Mitogens
Plant Lectins*
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Immunoglobulins; 0/Lectins; 0/Mitogens; 0/Plant Lectins; 0/jacalin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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