Document Detail


JUN, FOS, KROX, and CREB transcription factor proteins in the rat cortex: basal expression and induction by spreading depression and epileptic seizures.
MedLine Citation:
PMID:  8345107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The expression of the nuclear c-JUN, JUN B, JUN D, c-FOS, FOS B, KROX-24, and CREB transcription factors was investigated in the cortex of adult rats by immunocytochemistry. The expression patterns were studied in untreated rats and up to 24 hours following topical application of 1 M KCl to the cortical surface (KCl) or i.v. injection of bicuculline (BIC). Topical KCl induced cortical spreading depression and systemic injection of bicuculline evoked generalized tonic-clonic seizures. In untreated rats, JUN B, c-FOS, and FOS B were expressed in a small number of neurons in the piriform, perirhinal, entorhinal, and insular cortex and in layers II, III, and VI of all neocortical areas. In contrast, c-JUN, JUN D, and KROX-24 were expressed in all cortical layers but with different intensities of immunoreactivity (IR): c-JUN-IR was generally weak and predominantly present in layers II, III, and VI. JUN D-IR was equally strong in all layers. KROX-24 showed a prominent expression in layers II, IV, and VI. The CREB protein exhibited a slight preponderance in layer II and piriform cortex. Following KCl or BIC, a strong induction was seen for c-FOS, JUN B, and KROX-24, whereas c-JUN, JUN D, and FOS B showed only a moderate increase compared to basal levels. Changes of CREB-IR could not be detected. The localization of induced JUN, FOS, and KROX proteins reflected the pattern of labelling in untreated animals but demonstrated a higher intensity of labelling and an increased number of immunoreactive nuclei. The intensity and persistence of IR as well as the number of labelled cells following BIC exceeded those following KCl. Following BIC, increased levels of FOS B and JUN D were still present after 24 hours. Counterstaining with cresyl-violet and GFAP, a marker for astrocytes, revealed that JUN, FOS, and KROX proteins were expressed in neurons but not in glial cell populations. The present data demonstrate that CREB, JUN, FOS, and KROX transcription factors exhibit a layer-specific expression in the cerebral cortex with only slight area-specific differences both in untreated rats and following stimulation with KCl and BIC. The expression of transcription factor proteins indicate complex molecular genetic changes in cortical neurons due to pathophysiological events such as seizure activity and spreading depression.
Authors:
T Herdegen; J Sandkühler; P Gass; M Kiessling; R Bravo; M Zimmermann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  333     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-09-08     Completed Date:  1993-09-08     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  271-88     Citation Subset:  IM    
Affiliation:
II. Institute of Physiology, University of Heidelberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Activating Transcription Factor 1
Animals
Bicuculline
Cerebral Cortex / cytology,  metabolism*
Cortical Spreading Depression / physiology*
DNA-Binding Proteins / metabolism
Early Growth Response Protein 1
Epilepsy / chemically induced,  metabolism*
Gene Expression / drug effects
Genes, fos / genetics
Genes, jun / genetics
Immediate-Early Proteins*
Immunohistochemistry
Male
Neurons / metabolism
Potassium Chloride / pharmacology
Rats / metabolism*
Rats, Sprague-Dawley
Transcription Factors / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Activating Transcription Factor 1; 0/DNA-Binding Proteins; 0/Early Growth Response Protein 1; 0/Egr1 protein, rat; 0/Immediate-Early Proteins; 0/Transcription Factors; 485-49-4/Bicuculline; 7447-40-7/Potassium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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