| JNK1 and JNK2 differently regulate IL-12 production in THP-1 macrophage cells. | |
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MedLine Citation:
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PMID: 20483637 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Macrophages play a key role in initiating the innate responses to infection by secreting cytokines such as interleukin-12 (IL-12). This study defined the distinct regulation of lipopolysaccharide (LPS)-mediated IL-12 production by c-jun NH(2)-terminal kinase (JNK)1 and JNK2 isoforms in human macrophages. Knockdown of JNK1 and JNK2 by small interference RNA (siRNA) reduced and enhanced LPS-induced IL-12 p40 production in THP-1 macrophage cells, respectively. The simultaneous knockdown of JNK1 and JNK2 augmented LPS-induced IL-12 production as well as a specific JNK inhibitor. In addition, transfection of siRNA against phosphoinositide 3-kinase (PI3K) p110beta attenuated LPS-induced IL-12 production and JNK1 phosphorylation, while not affecting JNK2 phosphorylation. These findings indicate that JNK1- and JNK2-mediated signaling plays a positive and a negative role, respectively, in LPS-induced IL-12 production and PI3K p110beta controls LPS-induced JNK1 activation, not JNK2 activation, resulting in the positive regulation of IL-12 production in THP-1 macrophage cells. |
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Authors:
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Mitsuyoshi Utsugi; Kunio Dobashi; Akihiro Ono; Tamotsu Ishizuka; Takeshi Hisada; Yasuhiko Koga; Yasuo Shimizu; Tadayoshi Kawata; Shin-Ichi Matsuzaki; Haruka Aoki; Yosuke Kamide; Masatomo Mori |
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Publication Detail:
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Type: Journal Article Date: 2010-05-18 |
Journal Detail:
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Title: Cytokine Volume: 51 ISSN: 1096-0023 ISO Abbreviation: Cytokine Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-08 Completed Date: 2010-10-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9005353 Medline TA: Cytokine Country: United States |
Other Details:
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Languages: eng Pagination: 127-31 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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metabolism Anthracenes / pharmacology Cell Line Enzyme Activation Humans Interleukin-12 Subunit p40 / biosynthesis* Isoenzymes / metabolism Lipopolysaccharides / immunology Macrophages / immunology*, metabolism Mitogen-Activated Protein Kinase 8 / physiology* Mitogen-Activated Protein Kinase 9 / physiology* RNA, Small Interfering / pharmacology Signal Transduction |
| Chemical | |
Reg. No./Substance:
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0/Anthracenes; 0/IL12B protein, human; 0/Interleukin-12 Subunit p40; 0/Isoenzymes; 0/Lipopolysaccharides; 0/RNA, Small Interfering; 0/anthra(1,9-cd)pyrazol-6(2H)-one; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.1.24/Mitogen-Activated Protein Kinase 9; EC 2.7.11.24/Mitogen-Activated Protein Kinase 8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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