Document Detail


JKT-1 is not a human seminoma cell line.
MedLine Citation:
PMID:  17705808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The JKT-1 cell line has been used in multiple independent studies as a representative model of human testicular seminoma. However, no cell line for this specific tumour type has been independently confirmed previously; and therefore, the seminomatous origin of JKT-1 must be proven. The genetic constitution of the JKT-1 cells was determined using flow cytometry and spectral karyotyping, as well as array comparative genomic hybridization and fluorescent in situ hybridization. Marker profiling, predominantly based on differentially expressed proteins during normal germ cell development, was performed by immunohistochemistry and Western blot analyses. Moreover, genome wide affymetrix mRNA expression and profiling of 157 microRNAs was performed, and the status of genomic imprinting was determined. A germ cell origin of the JKT-1 cells was in line with genomic imprinting status and marker profile (including positive staining for several cancer-testis antigens). However, the supposed primary tumour, from which the cell line was derived, being indeed a classical seminoma, was molecularly proven not to be the origin of the cell line. The characteristic chromosomal anomalies of seminoma, e.g. gain of the short arm of chromosome 12, as well as the informative marker profile (positive staining for OCT3/4, NANOG, among others) were absent in the various JKT-1 cell lines investigated, irrespective of where the cells were cultured. All results indicate that the JKT-1 cell line is not representative of human seminoma. Although it can originate from an early germ cell, a non-germ cell derivation cannot be excluded.
Authors:
Jeroen de Jong; Hans Stoop; Ad J M Gillis; Ruud J H L M van Gurp; Ellen van Drunen; H Berna Beverloo; Yun-Fai Chris Lau; Dominik T Schneider; Jon K Sherlock; John Baeten; Shingo Hatakeyama; Chikara Ohyama; J Wolter Oosterhuis; Leendert H J Looijenga
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of andrology     Volume:  30     ISSN:  0105-6263     ISO Abbreviation:  Int. J. Androl.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-20     Completed Date:  2007-10-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000141     Medline TA:  Int J Androl     Country:  England    
Other Details:
Languages:  eng     Pagination:  350-65     Citation Subset:  IM    
Affiliation:
Department of Pathology, Erasmus MC-University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor*
Humans
Immunohistochemistry
Karyotyping
Male
Models, Biological
Reproducibility of Results
Seminoma*
Testicular Neoplasms*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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