| JC virus induces nonapoptotic cell death of human central nervous system progenitor cell-derived astrocytes. | |
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MedLine Citation:
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PMID: 15078969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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JC virus (JCV), a human neurotropic polyomavirus, demonstrates a selective glial cell tropism that causes cell death through lytic infection. Whether these cells die via apoptosis or necrosis following infection with JCV remains unclear. To investigate the mechanism of virus-induced cell death, we used a human central nervous system progenitor-derived astrocyte cell culture model developed in our laboratory. Using in situ DNA hybridization, immunocytochemistry, electron microscopy, and an RNase protection assay, we observed that astrocytes support a progressive JCV infection, which eventually leads to nonapoptotic cell death. Infected astrocyte cell cultures showed no difference from noninfected cells in mRNA expression of the caspase family genes or in any ultrastructural features associated with apoptosis. Infected cells demonstrated striking necrotic features such as cytoplasmic vacuolization, watery cytoplasm, and dissolution of organelles. Furthermore, staining for caspase-3 and terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling were not detected in infected astrocyte cultures. Our findings suggest that JCV-induced cell death of these progenitor cell-derived astrocytes does not utilize an apoptosis pathway but exhibits a pattern of cell destruction consistent with necrotic cell death. |
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Authors:
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Pankaj Seth; Frank Diaz; Jung-Hwa Tao-Cheng; Eugene O Major |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 78 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 2004 May |
Date Detail:
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Created Date: 2004-04-13 Completed Date: 2004-06-01 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: United States |
Other Details:
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Languages: eng Pagination: 4884-91 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Astrocytes
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pathology*,
virology* Cell Death Central Nervous System / cytology Humans In Situ Nick-End Labeling JC Virus / genetics, pathogenicity*, physiology Microscopy, Electron Necrosis Stem Cells / cytology Viral Structural Proteins / genetics, metabolism Virion / metabolism, ultrastructure Virus Replication |
| Chemical | |
Reg. No./Substance:
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0/Viral Structural Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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