Document Detail

JC-1, a sensitive probe for a simultaneous detection of P-glycoprotein activity and apoptosis in leukemic cells.
MedLine Citation:
PMID:  16568474     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: JC-1 probe has been successfully used for the analysis of either apoptosis or P-glycoprotein (P-gp) activity. Therefore, we wanted to see if JC-1 could also simultaneously assess both, P-gp activity and apoptosis, in acute myeloid leukemia (AML) cells. METHODS: P-gp activity was measured using JC-1 and compared to the results of the Rhodamine 123 (Rh 123) assay in P-gp negative and P-gp positive cell lines, and 12 AML samples. For apoptosis, spontaneous apoptosis, as well as, apoptosis induced by Cytosine Arabinosine and Homoharringtonine were analyzed. Both mitochondrial red fluorescence and cytoplasmic green fluorescence of JC-1 with and without a P-gp inhibitor (Cyclosporine A : CsA) were used for the identification of apoptotic cells, and this was compared to Annexin V/PI staining. RESULTS: (1) We found a good correlation between JC-1 and Rh 123 in viable cells. Even in a small population of viable cells, P-gp positive cells emitting low red fluorescence, gained on red fluorescence after P-gp inhibition with CsA permitting an evaluation of P-gp activity. (2) We found a good correlation between the Annexin V/PI staining and JC-1 (P < 0.0001) in the assessment of apoptotic cells. Most importantly, the apoptotic cells could be distinguished by the loss of red fluorescence and the increase of green fluorescence without any change after P-gp inhibition with CsA. CONCLUSIONS: JC-1 can simultaneously evaluate two important parameters involved in drug resistance in AML cells, P-gp activity and apoptosis.
Driss Chaoui; Anne-Marie Faussat; Patricia Majdak; Ruoping Tang; Jean-Yves Perrot; Sabine Pasco; Christophe Klein; Jean-Pierre Marie; Ollivier Legrand
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytometry. Part B, Clinical cytometry     Volume:  70     ISSN:  1552-4949     ISO Abbreviation:  Cytometry B Clin Cytom     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-04-25     Completed Date:  2006-07-10     Revised Date:  2009-12-11    
Medline Journal Info:
Nlm Unique ID:  101235690     Medline TA:  Cytometry B Clin Cytom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  189-96     Citation Subset:  IM    
Copyright Information:
Copyright 2006 International Society for Analytical Cytology.
Université Pierre et Marie Curie-Paris 6, UMR S 736, Les Cordeliers, France.
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MeSH Terms
Acute Disease
Annexin A5 / chemistry
Benzimidazoles / chemistry*
Carbocyanines / chemistry*
Cell Line, Tumor
Cyclosporine / pharmacology
Cytoplasm / chemistry,  drug effects
Flow Cytometry / methods*
Fluorescent Dyes / chemistry
Leukemia, Myeloid / metabolism,  pathology*
Mitochondria / chemistry,  drug effects
P-Glycoprotein / analysis*,  antagonists & inhibitors,  chemistry
Propidium / chemistry
Rhodamine 123 / chemistry
Staining and Labeling / methods
Tumor Cells, Cultured
Reg. No./Substance:
0/Annexin A5; 0/Benzimidazoles; 0/Carbocyanines; 0/Fluorescent Dyes; 0/P-Glycoprotein; 21527-78-6/5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine; 36015-30-2/Propidium; 59865-13-3/Cyclosporine; 62669-70-9/Rhodamine 123

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