| JAM4 enhances hepatocyte growth factor-mediated branching and scattering of Madin-Darby canine kidney cells. | |
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MedLine Citation:
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PMID: 15330858 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Junctional adhesion molecule (JAM) 4 is a member of immunoglobulin superfamily that interacts with MAGI-1, a membrane-associated guanylate kinase protein at tight junctions in epithelial cells. We prepared Madin-Darby canine kidney II (MDCK) cells expressing JAM4 (MDCK-JAM4) and compared them with wild MDCK cells. The treatment of hepatocyte growth factor (HGF) induced more prominent branching and scattering in MDCK-JAM4 cells. Subsequently we attempted to identify signalling pathways modified by JAM4. The over-expression of JAM4 induced the formation of protrusions in COS-7 cells. Although those protrusions were different from typical lamellipodia, the dominant negative mutant of Rac suppressed them. The pull-down assay using CDC42 and Rac interactive binding domain of PAK also supports that Rac is activated in COS-7 cells expressing JAM4. Taken together, JAM4 itself activates Rac and may augment Rac activation by HGF, resulting in the enhancement of branching and scattering. |
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Authors:
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Hiroki Mori; Susumu Hirabayashi; Madoka Shirasawa; Haruhiko Sugimura; Yutaka Hata |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Genes to cells : devoted to molecular & cellular mechanisms Volume: 9 ISSN: 1356-9597 ISO Abbreviation: Genes Cells Publication Date: 2004 Sep |
Date Detail:
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Created Date: 2004-08-27 Completed Date: 2004-10-18 Revised Date: 2011-12-22 |
Medline Journal Info:
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Nlm Unique ID: 9607379 Medline TA: Genes Cells Country: England |
Other Details:
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Languages: eng Pagination: 811-9 Citation Subset: IM |
Affiliation:
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Department of Medical Biochemistry, Graduate School of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals COS Cells Cell Adhesion Molecules / antagonists & inhibitors, metabolism, physiology* Cell Line Cell Movement Cercopithecus aethiops Dogs Epithelial Cells / cytology*, drug effects, metabolism Epithelium / embryology, growth & development, metabolism Hepatocyte Growth Factor / pharmacology* Kidney / embryology, growth & development, metabolism Mutation Pseudopodia / ultrastructure Rats Ureter / cytology, embryology, growth & development rac GTP-Binding Proteins / metabolism rac1 GTP-Binding Protein / genetics |
| Chemical | |
Reg. No./Substance:
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0/Cell Adhesion Molecules; 0/LOC304000 protein, rat; 67256-21-7/Hepatocyte Growth Factor; EC 3.6.5.2/rac GTP-Binding Proteins; EC 3.6.5.2/rac1 GTP-Binding Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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