Document Detail


The JAK2V617F allele burden and STAT3- and STAT5 phosphorylation in myeloproliferative neoplasms: early prefibrotic myelofibrosis compared with essential thrombocythemia, polycythemia vera and myelofibrosis.
MedLine Citation:
PMID:  21749449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Early prefibrotic myelofibrosis (early PMF) is a diagnosis that clinically and histologically mimic essential thrombocythemia (ET), but is important to distinguish from ET, polycythemia vera (PV) and primary myelofibrosis (PMF) due to its different prognosis and clinical evolution. In this study, we assessed the allele burden of JAK2V617F in bone marrow biopsies from patients with these chronic myeloproliferative neoplasms. We correlated our findings with the amount of phosphorylated STAT3 (P-STAT3) and STAT5 (P-STAT5) in megakaryocyte nuclei in the bone marrow. The JAK2V617F allele burden was significantly higher in patients with PV (median: 50.99, range: 23.08-97.29, p < 0.01 and p < 0.01) and PMF (median: 44.13, range: 33.61-92.17, p < 0.05 and p < 0.01) compared with a low allele burden in ET (median: 23.465, range: 8.67-47.92) and early PMF (median: 25.68, range: 0.61-49.13) respectively. In addition, we found a significantly higher phosphorylation of STAT5 and STAT3 in the JAK2V617F positive group than in the negative group. There was no positive correlation between increasing JAK2V617F allele burden and the amount of P-STAT3 and P-STAT5. However, we found low values of P-STAT5 in bone marrow biopsies from patients with ETJAK2V617F+ as compared with patients with early PMFJAK2V617F+. Although this difference was statistically significant, larger studies are needed to firmly support this conclusion.
Authors:
Malene Risum; Ann Madelung; Henrik Bondo; Michael Bzorek; Michael Holmsgaard Kristensen; Inger Merete Stamp; Hans Carl Hasselbalch
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2011-04-17
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  119     ISSN:  1600-0463     ISO Abbreviation:  APMIS     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-07-13     Completed Date:  2011-09-16     Revised Date:  2011-10-30    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  498-504     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. APMIS © 2011 APMIS.
Affiliation:
Department of Hematology, Roskilde Hospital, Denmark. malene.risum@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Alleles
Biopsy
Bone Marrow Neoplasms / enzymology,  genetics*,  pathology
Cross-Sectional Studies
DNA / chemistry,  genetics
Humans
Immunohistochemistry
Janus Kinase 2 / genetics*,  metabolism
Phosphorylation
Polycythemia Vera / enzymology,  genetics*,  pathology
Polymorphism, Single Nucleotide
Primary Myelofibrosis / enzymology,  genetics,  pathology
Retrospective Studies
STAT3 Transcription Factor / metabolism*
STAT5 Transcription Factor / metabolism*
Statistics, Nonparametric
Thrombocythemia, Essential / enzymology,  genetics*,  pathology
Chemical
Reg. No./Substance:
0/STAT3 Transcription Factor; 0/STAT3 protein, human; 0/STAT5 Transcription Factor; 9007-49-2/DNA; EC 2.7.10.1/Janus Kinase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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