| The JAK2V617F allele burden and STAT3- and STAT5 phosphorylation in myeloproliferative neoplasms: early prefibrotic myelofibrosis compared with essential thrombocythemia, polycythemia vera and myelofibrosis. | |
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MedLine Citation:
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PMID: 21749449 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Early prefibrotic myelofibrosis (early PMF) is a diagnosis that clinically and histologically mimic essential thrombocythemia (ET), but is important to distinguish from ET, polycythemia vera (PV) and primary myelofibrosis (PMF) due to its different prognosis and clinical evolution. In this study, we assessed the allele burden of JAK2V617F in bone marrow biopsies from patients with these chronic myeloproliferative neoplasms. We correlated our findings with the amount of phosphorylated STAT3 (P-STAT3) and STAT5 (P-STAT5) in megakaryocyte nuclei in the bone marrow. The JAK2V617F allele burden was significantly higher in patients with PV (median: 50.99, range: 23.08-97.29, p < 0.01 and p < 0.01) and PMF (median: 44.13, range: 33.61-92.17, p < 0.05 and p < 0.01) compared with a low allele burden in ET (median: 23.465, range: 8.67-47.92) and early PMF (median: 25.68, range: 0.61-49.13) respectively. In addition, we found a significantly higher phosphorylation of STAT5 and STAT3 in the JAK2V617F positive group than in the negative group. There was no positive correlation between increasing JAK2V617F allele burden and the amount of P-STAT3 and P-STAT5. However, we found low values of P-STAT5 in bone marrow biopsies from patients with ETJAK2V617F+ as compared with patients with early PMFJAK2V617F+. Although this difference was statistically significant, larger studies are needed to firmly support this conclusion. |
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Authors:
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Malene Risum; Ann Madelung; Henrik Bondo; Michael Bzorek; Michael Holmsgaard Kristensen; Inger Merete Stamp; Hans Carl Hasselbalch |
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Publication Detail:
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Type: Comparative Study; Journal Article Date: 2011-04-17 |
Journal Detail:
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Title: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica Volume: 119 ISSN: 1600-0463 ISO Abbreviation: APMIS Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-13 Completed Date: 2011-09-16 Revised Date: 2011-10-30 |
Medline Journal Info:
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Nlm Unique ID: 8803400 Medline TA: APMIS Country: Denmark |
Other Details:
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Languages: eng Pagination: 498-504 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. APMIS © 2011 APMIS. |
Affiliation:
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Department of Hematology, Roskilde Hospital, Denmark. malene.risum@gmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Biopsy Bone Marrow Neoplasms / enzymology, genetics*, pathology Cross-Sectional Studies DNA / chemistry, genetics Humans Immunohistochemistry Janus Kinase 2 / genetics*, metabolism Phosphorylation Polycythemia Vera / enzymology, genetics*, pathology Polymorphism, Single Nucleotide Primary Myelofibrosis / enzymology, genetics, pathology Retrospective Studies STAT3 Transcription Factor / metabolism* STAT5 Transcription Factor / metabolism* Statistics, Nonparametric Thrombocythemia, Essential / enzymology, genetics*, pathology |
| Chemical | |
Reg. No./Substance:
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0/STAT3 Transcription Factor; 0/STAT3 protein, human; 0/STAT5 Transcription Factor; 9007-49-2/DNA; EC 2.7.10.1/Janus Kinase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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