Document Detail

JAK2 and AMP-kinase inhibition in vitro by food extracts, fractions and purified phytochemicals.
MedLine Citation:
PMID:  25463744     Owner:  NLM     Status:  Publisher    
We have identified a range of food phytochemicals that inhibit Janus Kinase 2 (JAK2) and Adenosine Monophosphate Kinase (AMPK). A mutated and dysregulated form of JAK2, a tyrosine kinase, is associated with several diseases including Crohn's disease. Using an in vitro, time-resolved fluorescence (TR-FRET) assay, we tested 49 different types of food extracts, plus 10 concentrated fractions of increasing hydrophobicity from each extract, to find foods containing JAK2 inhibitors. The food extracts tested included grains, meat, fish, shellfish, dairy products, herbs, mushrooms, hops, fruits and vegetables. Several fruits were potent inhibitors of JAK2: blackberry, boysenberry, feijoa, pomegranate, rosehip and strawberry, which all contain ellagitannins, known inhibitors of kinases. These fruits are in the Rosales and Myrtales plant orders. No other foods gave >1% of the maximal JAK2 inhibitory activities of these fruits. AMPK, a sensor and regulator of energy metabolism in cells, is a serine-threonine kinase which is reported to be activated by various flavonoid phytochemicals. Using a TR-FRET assay, we tested various fruit extracts for AMPK activation and inhibition. Ellagitannin containing foods scored highly as AMPK inhibitors. Despite several reports of AMPK activation in whole cells by phytochemicals, no extracts or pure compounds activated AMPK in our assay.
Harry Martin; Elaine J Burgess; Wendy A Smith; Tony K McGhie; Janine M Cooney; Rona C M Lunken; Erika de Guzman; Tania Trower; Nigel B Perry
Related Documents :
25083994 - Pparγ ablation sensitizes proopiomelanocortin neurons to leptin during high-fat feeding.
25260774 - Energy and nutritional value of the meals in kindergartens in niš (serbia).
25164604 - Qualitative study exploring healthy eating practices and physical activity among adoles...
25373174 - Entomocidal effects of beech apricot, labramia bojeri, seed extract on a soybean pest, ...
2463794 - A technique for spot application of fibrin glue during open heart operations.
15250844 - The effect of practical portion size measurement aids on the accuracy of portion size e...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-12-2
Journal Detail:
Title:  Food & function     Volume:  -     ISSN:  2042-650X     ISO Abbreviation:  Food Funct     Publication Date:  2014 Dec 
Date Detail:
Created Date:  2014-12-2     Completed Date:  -     Revised Date:  2014-12-3    
Medline Journal Info:
Nlm Unique ID:  101549033     Medline TA:  Food Funct     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Adverse Structural Remodeling of the Left Ventricle and Ventricular Arrhythmias in Patients with Dep...
Next Document:  Vibrational energy transfer dynamics in ruthenium polypyridine transition metal complexes.