| A J-multiplied HMQC (MJ-HMQC) experiment for measuring (3)J(HNHalpha) coupling constants. | |
| | |
MedLine Citation:
|
PMID: 10968977 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The J-multiplied HSQC experiment (MJ-HSQC: S. Heikkinen et al., J. Magn. Reson 137, 243 (1999)) amplifies J coupling constants m times and allows direct observation of the (3)J(HNHalpha) coupling constants of peptides and proteins (<10 kDa). The drawbacks to this method are line broadening in the f(1)-dimension and lower sensitivity. In the J-multiplied HMQC (MJ-HMQC) experiment described here, the PEP-HSQC pulse sequence is replaced by a sensitivity-enhanced HMQC section, and the total decay time for the J-coupling and the chemical shift evolution is shortened by a period of t(1). This experiment affords narrower linewidth and enhances the sensitivity by 34%, on an average of 105 well-isolated peaks, when compared with the MJ-HSQC experiment. |
| | |
Authors:
|
Y Xia; X Kong; N Ip; G Zhu |
Related Documents
:
|
21997677 - A new incremental test for vo(2max) accurate measurement by increasing vo(2max) plateau... 1025637 - The effects of haemorrhagic anaemia and physical training on the energy metabolism and ... 21951697 - The effect of age and tongue exercise on bdnf and trkb in the hypoglossal nucleus of rats. 22116577 - Accelerometry-based physical activity and exercise capacity in pediatric kidney transpl... 19339657 - Exercise training and cardiac rehabilitation in primary and secondary prevention of cor... 18355977 - Diffusion-weighted magnetic resonance imaging to evaluate major salivary gland function... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of magnetic resonance (San Diego, Calif. : 1997) Volume: 146 ISSN: 1090-7807 ISO Abbreviation: J. Magn. Reson. Publication Date: 2000 Sep |
Date Detail:
|
Created Date: 2000-10-25 Completed Date: 2000-10-25 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9707935 Medline TA: J Magn Reson Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 228-31 Citation Subset: IM |
Copyright Information:
|
Copyright 2000 Academic Press. |
Affiliation:
|
Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Algorithms Magnetic Resonance Spectroscopy / methods* Nitrogen Isotopes Peptides / chemistry* Proteins / chemistry* Receptor, Ciliary Neurotrophic Factor / chemistry |
| Chemical | |
Reg. No./Substance:
|
0/Nitrogen Isotopes; 0/Peptides; 0/Proteins; 0/Receptor, Ciliary Neurotrophic Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Single-coil surface imaging using a radiofrequency field gradient
Next Document: 2J,(3)J-HMBC: A new long-range heteronuclear shift correlation technique capable of differentiating ...