Document Detail


Iterons of stringently controlled plasmids and DNA fingerprinting.
MedLine Citation:
PMID:  8400717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DNA probes which detect polymorphic, repetitive sequences in a variety of genomes have been developed using different approaches. Naturally occurring plasmids, with repeated units termed iterons near or within their origins (ori) or replication, could be of interest for the development of probes, possibly even revealing novel minisatellite families in mammals involved in replicational processes. We used the plasmids P1, pSC101 and RSF1010 or their PCR amplified ori regions as probes in Southern blot hybridisations with mammalian DNA. At low stringency they generated reproducible fingerprint-like patterns. A bovine genomic library was screened at the same stringency with the PCR-amplified ori region of P1 containing the five times repeated core sequence 5'-ATGTGTGNTGNNGGG-3' to generate a probe for cattle DNA with higher specificity.
Authors:
K J Huebscher; G Dolf; J Frey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  EXS     Volume:  67     ISSN:  1023-294X     ISO Abbreviation:  EXS     Publication Date:  1993  
Date Detail:
Created Date:  1993-11-05     Completed Date:  1993-11-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9204529     Medline TA:  EXS     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  79-85     Citation Subset:  IM    
Affiliation:
Institute of Animal Breeding, University of Berne, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cattle
DNA / genetics
DNA Fingerprinting*
DNA Probes*
DNA, Satellite / genetics
Gene Library
Humans
Male
Molecular Sequence Data
Perissodactyla
Repetitive Sequences, Nucleic Acid*
Species Specificity
Chemical
Reg. No./Substance:
0/DNA Probes; 0/DNA, Satellite; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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