Document Detail


Iterative design of peptide-based hydrogels and the effect of network electrostatics on primary chondrocyte behavior.
MedLine Citation:
PMID:  22841922     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Iterative peptide design was used to generate two peptide-based hydrogels to study the effect of network electrostatics on primary chondrocyte behavior. MAX8 and HLT2 peptides have formal charge states of +7 and +5 per monomer, respectively. These peptides undergo triggered folding and self-assembly to afford hydrogel networks having similar rheological behavior and local network morphologies, yet different electrostatic character. Each gel can be used to directly encapsulate and syringe-deliver cells. The influence of network electrostatics on cell viability after encapsulation and delivery, extracellular matrix deposition, gene expression, and the bulk mechanical properties of the gel-cell constructs as a function of culture time was assessed. The less electropositive HLT2 gel provides a microenvironment more conducive to chondrocyte encapsulation, delivery, and phenotype maintenance. Cell viability was higher for this gel and although a moderate number of cells dedifferentiated to a fibroblast-like phenotype, many retained their chondrocytic behavior. As a result, gel-cell constructs prepared with HLT2, cultured under static in vitro conditions, contained more GAG and type II collagen resulting in mechanically superior constructs. Chondrocytes delivered in the more electropositive MAX8 gel experienced a greater degree of cell death during encapsulation and delivery and the remaining viable cells were less prone to maintain their phenotype. As a result, MAX8 gel-cell constructs had fewer cells, of which a limited number were capable of laying down cartilage-specific ECM.
Authors:
Chomdao Sinthuvanich; Lisa A Haines-Butterick; Katelyn J Nagy; Joel P Schneider
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2012-07-28
Journal Detail:
Title:  Biomaterials     Volume:  33     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-08-13     Completed Date:  2012-12-18     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  7478-88     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Ltd.
Affiliation:
Chemical Biology Laboratory, National Cancer Institute, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cattle
Cell Shape / drug effects
Cell Survival / drug effects
Cells, Cultured
Chondrocytes / cytology*,  drug effects,  metabolism
Elastic Modulus / drug effects
Extracellular Matrix / drug effects,  metabolism
Extracellular Matrix Proteins / genetics,  metabolism
Gene Expression Regulation / drug effects
Glycosaminoglycans / metabolism
Hydrogels / chemistry*
Molecular Sequence Data
Peptides / chemistry*,  pharmacology
Rheology / drug effects
Static Electricity*
Grant Support
ID/Acronym/Agency:
ZIA BC011313-02/BC/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Extracellular Matrix Proteins; 0/Glycosaminoglycans; 0/Hydrogels; 0/Peptides
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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