| Itaconic acid is a mammalian metabolite induced during macrophage activation. | |
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MedLine Citation:
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PMID: 21919507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Itaconic acid (ITA), or methylenesuccinic acid, is not generally classified as a mammalian metabolite. Using NMR-based metabolomics and (13)C-labeling, we have detected ITA in both macrophage-like VM-M3 and RAW 264.7 tumor cell lines as well as stimulated and unstimulated primary murine macrophages. Macrophage activation by addition of lipopolysaccharide and IFN-γ markedly increased ITA production and secretion. Crude cell extracts synthesize ITA via decarboxylation of cis-aconitate, indicative of a novel mammalian cis-aconitic decarboxylase activity. Our results highlight a previously unidentified biosynthetic pathway related to TCA cycle metabolism in mammalian cells and a novel metabolite that likely plays a role in macrophage-based immune response. |
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Authors:
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Cheryl L Strelko; Wenyun Lu; Fay J Dufort; Thomas N Seyfried; Thomas C Chiles; Joshua D Rabinowitz; Mary F Roberts |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-09-27 |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 133 ISSN: 1520-5126 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-12 Completed Date: 2012-02-03 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 16386-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line, Tumor Interferon-gamma / pharmacology Lipopolysaccharides / pharmacology Macrophages / drug effects, metabolism* Mice Succinates / analysis, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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1RC1 CA147961/CA/NCI NIH HHS; RC1 CA147961-02/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 0/Succinates; 82115-62-6/Interferon-gamma; 97-65-4/itaconic acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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