| Isoxyl particles for pulmonary delivery: In vitro cytotoxicity and potency. | |
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MedLine Citation:
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PMID: 20600722 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pulmonary delivery of isoxyl may increase drug efficacy by targeting alveolar macrophages which are host cells for Mycobacteria. Isoxyl microparticles (1-2microm) were obtained by antisolvent precipitation and simultaneous spray drying method. The controls were made by mixing isoxyl solution in DMSO with cell culture media. Depending on the drug concentration, either isoxyl solution or nanosuspension was obtained in these controls. In the study, MTT (methylthiazol tetrazolium) and LDH (lactose dehydrogenase) assays were utilized to test cytotoxicity of these particle suspensions or solutions toward macrophages. Isoxyl microparticles and controls in concentrations up to 100microg/ml were not toxic to macrophages. Both isoxyl microparticle suspensions and controls showed bactericidal activity, as estimated by death of mycobacteria inside the macrophages, at a concentration of 5microg/ml. |
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Authors:
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Chenchen Wang; Anthony J Hickey |
Publication Detail:
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Type: Journal Article Date: 2010-06-25 |
Journal Detail:
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Title: International journal of pharmaceutics Volume: 396 ISSN: 1873-3476 ISO Abbreviation: Int J Pharm Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-26 Completed Date: 2010-11-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7804127 Medline TA: Int J Pharm Country: Netherlands |
Other Details:
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Languages: eng Pagination: 99-104 Citation Subset: IM |
Copyright Information:
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Copyright 2010. Published by Elsevier B.V. |
Affiliation:
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Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Division of Molecular Pharmaceutics, Campus Box #7571, 1310 Kerr Hall, Chapel Hill, NC 27599, United States. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Inhalation Antitubercular Agents / administration & dosage, chemistry, pharmacology*, toxicity Cell Line Cell Survival / drug effects Chemical Precipitation Chemistry, Pharmaceutical Colony Count, Microbial Dimethyl Sulfoxide / chemistry Dose-Response Relationship, Drug Drug Compounding Humans Macrophages / drug effects, microbiology*, pathology Microbial Sensitivity Tests Mycobacterium bovis / drug effects*, growth & development Nanoparticles Particle Size Phenylthiourea / administration & dosage, analogs & derivatives*, chemistry, pharmacology, toxicity Powders Solvents / chemistry Technology, Pharmaceutical / methods |
| Chemical | |
Reg. No./Substance:
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0/Antitubercular Agents; 0/Powders; 0/Solvents; 103-85-5/Phenylthiourea; 67-68-5/Dimethyl Sulfoxide; 910-86-1/thiocarlide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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