Document Detail

Isovolumetric regulation of rat glial cells during development and correction of hypo-osmolality.
MedLine Citation:
PMID:  10822139     Owner:  NLM     Status:  MEDLINE    
Rat C6 glioma cells undergo regulatory volume decrease (RVD) following sudden exposure to hypo-osmolality, but little or no regulatory volume increase (RVI) is observed when cells cultured in hypo-osmotic media are suddenly returned to isoosmolality. Because C6 glioma cells would rarely be exposed to sudden large changes in osmolality in vivo, we examined the ability of these cells to maintain their volume, termed 'isovolumetric regulation', when exposed to gradual changes in osmolality. When osmolality was gradually reduced by reduction of NaCl concentration from 300 to 250 mOsmol/kg at a rate of 0.4 mOsmol/kg/min or less cells were able to maintain their volume, while at higher rates, the cells swelled. Cells which were cultured in hypo-osmotic (200 mOsmol/kg) media for 3 days exhibited isovolumetric regulation at rates of osmolality increase of 0.5 mOsmol/kg/min or less over the range of 200-250 mOsmol/kg. We conclude that rat C6 glioma cells can sensitively regulate their volume over the osmolality range of pathophysiologic interest at rates of osmolality change which are faster than those generally seen in clinical conditions.
J W Lohr; L Yohe
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Neuroscience letters     Volume:  286     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-07-14     Completed Date:  2000-07-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  5-8     Citation Subset:  IM    
Departments of Medicine and Pharmacology, State University of New York at Buffalo, NY 14215, Buffalo, USA.
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MeSH Terms
Brain / cytology,  drug effects,  metabolism
Cell Size / drug effects*,  physiology*
Culture Media / pharmacology*
Hypotonic Solutions / pharmacology*
Neuroglia / drug effects*,  metabolism*
Osmolar Concentration
Sodium Chloride / metabolism
Time Factors
Tumor Cells, Cultured
Reg. No./Substance:
0/Culture Media; 0/Hypotonic Solutions; 7647-14-5/Sodium Chloride

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