| Isovaleryl-homoserine lactone, an unusual branched-chain quorum-sensing signal from the soybean symbiont Bradyrhizobium japonicum. | |
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MedLine Citation:
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PMID: 21949379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Many species of Proteobacteria communicate by using LuxI-LuxR-type quorum-sensing systems that produce and detect acyl-homoserine lactone (acyl-HSL) signals. Most of the known signals are straight-chain fatty acyl-HSLs, and evidence indicates that LuxI homologs prefer fatty acid-acyl carrier protein (ACP) over fatty acyl-CoA as the acyl substrate for signal synthesis. Two related LuxI homologs, RpaI and BtaI from Rhodopseudomonas palustris and photosynthetic stem-nodulating bradyrhizobia, direct production of the aryl-HSLs p-coumaroyl-HSL and cinnamoyl-HSL, respectively. Here we report that BjaI from the soybean symbiont Bradyrhizobium japonicum USDA110 is closely related to RpaI and BtaI and catalyzes the synthesis of isovaleryl-HSL (IV-HSL), a branched-chain fatty acyl-HSL. We show that IV-HSL induces expression of bjaI, and in this way IV-HSL functions like many other acyl-HSL quorum-sensing signals. Purified histidine-tagged BjaI was an IV-HSL synthase, which was active with isovaleryl-CoA but not detectably so with isovaleryl-ACP. This suggests that the RpaI-BtaI-BjaI subfamily of acyl-HSL synthases may use CoA- rather than ACP-linked substrates for acyl-HSL synthesis. The bjaI-linked bjaR(1) gene is involved in the response to IV-HSL, and BjaR(1) is sensitive to IV-HSL at concentrations as low as 10 pM. Low but sufficient levels of IV-HSL (about 5 nM) accumulate in B. japonicum culture fluid. The low levels of IV-HSL synthesis have likely contributed to the fact that the quorum-sensing signal from this bacterium has not been described elsewhere. |
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Authors:
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Andrea Lindemann; Gabriella Pessi; Amy L Schaefer; Margrith E Mattmann; Quin H Christensen; Aline Kessler; Hauke Hennecke; Helen E Blackwell; E Peter Greenberg; Caroline S Harwood |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-09-26 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-05 Completed Date: 2011-12-13 Revised Date: 2012-04-04 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 16765-70 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of Washington, Seattle, WA 98195, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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4-Butyrolactone
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analogs & derivatives*,
metabolism Acyl Coenzyme A / metabolism Amino Acid Sequence Bacterial Proteins / genetics, metabolism Bradyrhizobium / metabolism*, physiology Cluster Analysis Computational Biology Gene Expression Regulation, Bacterial / physiology* Phylogeny Quorum Sensing / physiology* Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Soybeans / microbiology* Transcription Factors / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AI063326/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acyl Coenzyme A; 0/Bacterial Proteins; 0/LuxI protein, Bacteria; 0/Transcription Factors; 1192-20-7/homoserine lactone; 6244-91-3/isovaleryl-coenzyme A; 96-48-0/4-Butyrolactone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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