Document Detail


Isotretinoin alters morphology, polarity, and motility of neural crest cells in culture.
MedLine Citation:
PMID:  10080299     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Migrating neural crest cells (NCCs) contribute to a diverse array of vertebrate head and neck structures. Retinoids are proven human and animal teratogens. To elucidate isotretinoin's effects, cranial and trunk neural folds were microdissected from chick embryos and cultured. Image analysis and immunostaining were used to quantitate cell behavior. We found that a higher proportion of Stage 8, 9, and 10 treated NCCs were rounded and clustered. Medians and means for cell area, perimeter, and elongation index were lower for treated cells from Stage 9 and 10 embryos, but not from Stage 8. Cumulative medians and means for changes in area and perimeter, and cell migration were similarly lower. Thus interference with the transitory basal activity of the cytoskeleton that adjusts and determines cell-substratum adhesion, spreading, elongation, and migration may be the mechanism by which isotretinoin acts on NCCs in slightly older embryos.
Authors:
A S Orris; L C Banicky; D J Wiens
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Reproductive toxicology (Elmsford, N.Y.)     Volume:  13     ISSN:  0890-6238     ISO Abbreviation:  Reprod. Toxicol.     Publication Date:    1999 Jan-Feb
Date Detail:
Created Date:  1999-05-11     Completed Date:  1999-05-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8803591     Medline TA:  Reprod Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  45-52     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Northern Iowa, Cedar Falls 50614, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / drug effects
Cell Movement / drug effects*
Cell Polarity / drug effects*
Cell Size / drug effects
Cells, Cultured
Chick Embryo
Image Processing, Computer-Assisted
Isotretinoin / toxicity*
Neural Crest / cytology,  drug effects*
Teratogens / toxicity*
Grant Support
ID/Acronym/Agency:
N01-HD-2-3144/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Teratogens; 4759-48-2/Isotretinoin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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