Document Detail


Isotope dilution gas chromatographic-mass spectrometric method for the determination of lignans and isoflavonoids in human urine, including identification of genistein.
MedLine Citation:
PMID:  1663006     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe an isotope dilution gas chromatographic-mass spectrometric method for the quantitative determination of the lignans enterolactone, enterodiol and matairesinol and the isoflavonoids daidzein, equol, O-desmethylangolensin and genistein in urine. Furthermore we present the gas chromatographic/mass spectrometer identification of genistein. Urine samples were extracted on Sep-Pak cartridges, conjugated fractions were isolated by chromatography on the acetate form of DEAE-Sephadex and deuterated internal standards of all seven compounds were added to the samples before hydrolysis. The hydrolysate was extracted on a Sep-Pak cartridge and following chromatography on the acetate form of QAE-Sephadex two fractions were obtained: Fraction 1 contained equol, enterolactone, enterodiol, matairesinol and all estrogens and fraction 2 contained O-desmethylangolensin, daidzein and genistein. The latter was ready for gas chromatography/mass spectrometry, but the first one was further purified to eliminate the estrogens by chromatography on the carbonate form of QAE-Sephadex. Following silylation, the samples were analyzed by combined capillary column gas chromatography/mass spectrometry in the selective ion monitoring mode. The within-assay imprecision varied from 0.8-15.2% (mean 8.7%) and the between-assay imprecision from 4.1-13.9% (mean 9.3%), depending on compound and concentration level. The mean recovery of authentic standards added to urine extracts before hydrolysis varied from 96.6 to 105.5%. Values obtained from 10 Finnish omnivorous men are presented. Individual values for matairesinol (excretion range 3.3-59.9 nmol/24 h) and genistein (range 21.8-1180 nmol/24 h) in human urine have never been published before.
Authors:
H Adlercreutz; T Fotsis; C Bannwart; K Wähälä; G Brunow; T Hase
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  199     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1992-02-27     Completed Date:  1992-02-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  263-78     Citation Subset:  IM    
Affiliation:
Department of Clinical Chemistry, University of Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
4-Butyrolactone / analogs & derivatives,  urine
Butylene Glycols / urine
Chromans / urine
Female
Furans / urine
Gas Chromatography-Mass Spectrometry
Genistein
Humans
Indicator Dilution Techniques
Isoflavones / chemistry,  urine*
Lignans
Lignin / analysis*
Magnetic Resonance Spectroscopy
Male
Chemical
Reg. No./Substance:
0/Butylene Glycols; 0/Chromans; 0/Furans; 0/Isoflavones; 0/Lignans; 21255-69-6/O-desmethylangolensin; 446-72-0/Genistein; 486-66-8/daidzein; 531-95-3/equol; 580-72-3/matairesinol; 76543-15-2/2,3-bis(3'-hydroxybenzyl)butyrolactone; 76543-16-3/2,3-bis(3'-hydroxybenzyl)butane-1,4-diol; 9005-53-2/Lignin; 96-48-0/4-Butyrolactone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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