Document Detail


Isotonic biaxial loading of fibroblast-populated collagen gels: a versatile, low-cost system for the study of mechanobiology.
MedLine Citation:
PMID:  14586707     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We developed a simple, versatile system for applying a range of biaxial loads to cell-matrix constructs for the study of mechanobiology. The system consists of porous polyethylene bars that are polymerized into a square fibroblast-populated gel and loaded by freely hanging weights attached to sutures routed through a custom loading rig. The cost to manufacture each mold/loading rig pair was less than US dollars 250 and the expected life of the components is up to 10 years. Neonatal and adult cardiac fibroblasts contracted gels to a decreasing extent as external load was increased ( P=0.003) and achieved contraction forces of up to 1.4 mN per million cells. Strain distributions were reasonably homogeneous in the central region of the gel (25% of gel area), but clearly nonhomogeneous outside that central region. The primary advantages of this system are simplicity, low cost, biaxial loading, and the ability to test for a dose-response effect of mechanical load. The current disadvantages are the inability to apply cycling loading and the inhomogeneities introduced by the use of rigid loading bars.
Authors:
V Knezevic; A J Sim; T K Borg; J W Holmes
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biomechanics and modeling in mechanobiology     Volume:  1     ISSN:  1617-7959     ISO Abbreviation:  Biomech Model Mechanobiol     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2003-11-05     Completed Date:  2004-01-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101135325     Medline TA:  Biomech Model Mechanobiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  59-67     Citation Subset:  IM; S    
Affiliation:
Department of Biomedical Engineering, Columbia University, 1210 Amsterdam Ave, New York, NY 10027, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Anisotropy
Cell Culture Techniques / economics,  instrumentation*,  methods
Cells, Cultured
Equipment Design
Equipment Failure Analysis*
Fibroblasts / physiology*
Gels
Heart / physiology
Membranes, Artificial*
Physical Stimulation / instrumentation*,  methods*
Rats
Rats, Sprague-Dawley
United States
Weight-Bearing / physiology*
Grant Support
ID/Acronym/Agency:
R01 HL-37669/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Gels; 0/Membranes, Artificial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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