Document Detail

Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review.
MedLine Citation:
PMID:  22592150     Owner:  NLM     Status:  MEDLINE    
Nucleic Acid Testing (NAT) promises rapid, sensitive and specific diagnosis of infectious, inherited and genetic disease. The next generation of diagnostic devices will interrogate the genetic determinants of such conditions at the point-of-care, affording clinicians prompt reliable diagnosis from which to guide more effective treatment. The complex biochemical nature of clinical samples, the low abundance of nucleic acid targets in the majority of clinical samples and existing biosensor technology indicate that some form of nucleic acid amplification will be required to obtain clinically relevant sensitivities from the small samples used in point-of-care testing (POCT). This publication provides an overview and thorough review of existing technologies for nucleic acid amplification. The different methods are compared and their suitability for POCT adaptation are discussed. Current commercial products employing isothermal amplification strategies are also investigated. In conclusion we identify the factors impeding the integration of the methods discussed in fully automated, sample-to-answer POCT devices.
Pascal Craw; Wamadeva Balachandran
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2012-05-16
Journal Detail:
Title:  Lab on a chip     Volume:  12     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-22     Completed Date:  2012-10-19     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  2469-86     Citation Subset:  IM    
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MeSH Terms
DNA Helicases / metabolism
DNA-Directed DNA Polymerase / genetics,  metabolism
Nucleic Acid Amplification Techniques / instrumentation,  methods*
Nucleic Acids / analysis*
Point-of-Care Systems
Recombinant Proteins / genetics,  metabolism
Grant Support
G0901608//Medical Research Council; //Medical Research Council; //Wellcome Trust
Reg. No./Substance:
0/Nucleic Acids; 0/Recombinant Proteins; EC DNA Polymerase; EC 3.6.4.-/DNA Helicases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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