| Isoprenoid metabolism is required for stimulation of the respiratory burst oxidase of HL-60 cells. | |
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MedLine Citation:
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PMID: 1310693 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The formation of oxygen radicals by phagocytic cells occurs through the activation of a multiple-component NADPH oxidase system. An unidentified low molecular weight GTP-binding protein has been proposed to modulate the activity of the NADPH oxidase. The low molecular weight GTP-binding proteins undergo posttranslational processing, including an initial covalent incorporation of an isoprenyl group. To test whether such an isoprenylation reaction might be required for the activity of the oxidase, we utilized compactin and lovastatin as inhibitors of the isoprenylation pathway. Treatment of DMSO-differentiated HL-60 cells with compactin produced a concentration-dependent inhibition of O2- formation in response to FMLP or phorbol myristate acetate. Cell viability was not affected nor was normal differentiation of the HL-60 cells into a neutrophil-like cell. The inhibitory effect of compactin was specifically prevented by addition of exogenous mevalonic acid to the HL-60 cells, indicating that the inhibitory effects of the drug were due to blockade of the pathway leading to isoprenoid synthesis. Addition of cholesterol, ubiquinone, or dolichol, which are also downstream products of the isoprenoid pathway, did not override the inhibitory effects of the drug. Subcellular fractions were prepared from compactin-treated cells, and the location of the compactin-sensitive factor was determined by complementation analysis in a cell-free NADPH oxidase system. The inhibited factor was localized to the HL-60 cytosol. These data suggest that an isoprenoid pathway intermediate is necessary for activation of the phagocyte NADPH oxidase. This is likely to represent the requirement for an isoprenoid moiety in the posttranslational modification of a low molecular weight GTP-binding protein. Our studies provide support for the involvement of such a low molecular weight GTP-binding protein in NADPH oxidase activation. |
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Authors:
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G M Bokoch; V Prossnitz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 89 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 1992 Feb |
Date Detail:
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Created Date: 1992-03-18 Completed Date: 1992-03-18 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 402-8 Citation Subset: AIM; IM |
Affiliation:
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Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Butadienes
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metabolism* Enzyme Activation GTP-Binding Proteins / metabolism Hemiterpenes* Humans Leukemia, Promyelocytic, Acute / metabolism Lovastatin / analogs & derivatives, pharmacology Mevalonic Acid / pharmacology NADH, NADPH Oxidoreductases / analysis* NADPH Oxidase Pentanes* Respiratory Burst / physiology* Superoxides / metabolism* Terpenes / metabolism* Tetradecanoylphorbol Acetate / pharmacology Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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GM-39434/GM/NIGMS NIH HHS; HL48008/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Butadienes; 0/Hemiterpenes; 0/Pentanes; 0/Terpenes; 11062-77-4/Superoxides; 150-97-0/Mevalonic Acid; 16561-29-8/Tetradecanoylphorbol Acetate; 73573-88-3/compactin; 75330-75-5/Lovastatin; 78-79-5/isoprene; EC 1.6.-/NADH, NADPH Oxidoreductases; EC 1.6.3.1/NADPH Oxidase; EC 3.6.1.-/GTP-Binding Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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