| Isoprenoid, lipid and protein contents in intact plastids isolated from mesocarp cells of traditional and high-pigment tomato cultivars at different ripening stages. | |
| | |
MedLine Citation:
|
PMID: 22264157 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
This study reports quali-quantitative analyses on isoprenoids, phospholipids, neutral lipids, phytosterols and proteins in purified plastids isolated from fresh fruits of traditional (Donald and Incas) and high-pigment (Kalvert and HLY-18) tomato cultivars (cvs) at four ripening stages. In all the investigated cvs, lycopene, β-catotene, lutein and total carotenoids varied significantly during ripening. Chromoplasts of red-ripe tomato fruits of high-pigment cvs accumulated twice as much as lycopene (307.6 and 319.2 μg/mg of plastid proteins in Kalvert and HLY-18, respectively) than ordinary cvs (178.6 and 151.7 μg/mg of plastid proteins in Donald and Incas, respectively), differences in chlorophyll and α-tocopherol contents were also evidenced. Phospholipids and phytosterols increased during ripening, whereas triglycerides showed a general decrease. Regardless the stage of ripening, palmitic acid was the major fatty acid in all cvs (ranging from 35 to 52% of the total fatty acids), followed by stearic, oleic, linoleic, linolenic and miristic acids, but their relative percentage was affected by ripening. Most of the bands detected on the SDS-PAGEs of plastid proteins were constantly present during chloroplast-to-chromoplast conversion, some others disappeared, only one, with a molecular weight of ~41.6 kDa, was found to increase in intensity. |
| | |
Authors:
|
Marcello Salvatore Lenucci; Lucia Serrone; Monica De Caroli; Paul D Fraser; Peter Michael Bramley; Gabriella Piro; Giuseppe Dalessandro |
Related Documents
:
|
20410017 - Distinct gene expression profiles characterize cellular responses to palmitate and oleate. 3665357 - Increased arachidonic acid levels in phospholipids of human colonic mucosa in inflammat... 1249447 - Growth requirements and lipid metabolism of pityrosporum orbiculare. 19654457 - Sphingomyelins in four ascidians, ciona intestinalis, halocynthia roretzi, halocynthia ... 21394467 - Down-regulation of the myo-inositol oxygenase gene family has no effect on cell wall co... 15009737 - Involvement of photooxidation of melanogenic precursors in prolonged pigmentation induc... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-23 |
Journal Detail:
|
Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Intramedullary pyogenic abscess in the conus medullaris.
Next Document: The Principles and Applications of Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS): A ...