Document Detail

Isopentenoid synthesis in isolated embryonic Drosophila cells. Farnesol catabolism and omega-oxidation.
MedLine Citation:
PMID:  3335546     Owner:  NLM     Status:  MEDLINE    
Kc cells divert minimally 40% of their mevalonate carbon to n-fatty acids and unidentified compounds covalently linked to macromolecules (Havel, C., Rector, E. R., II, and Watson, J. A. (1986) J. Biol. Chem. 261, 10150-10156). Furthermore mevalonate carbon diversion appears to occur at the polyprenyl 1-pyrophosphate level. This report summarizes initial efforts to define the mevalonate carbon diversion pathway. We demonstrate that Kc cell extracts readily metabolize [14C]farnesyl 1-pyrophosphate and [14C]farnesol, via common intermediates, to identical 14C-products. Two of the major 14C-products were identified as trans,trans-3,7,11-trimethyl-2,6,10-dodecatrien-1,12-dioic acid and trans-3,7-dimethyl-2,6-decadien-1,10-dioic acid. Similar acids were also synthesized by supplemented rat liver extracts incubated with [14C]farnesol. We conclude that (a) mevalonate carbon diversion at the level of polyprenyl 1-pyrophosphate is a viable metabolic strategy, (b) polyprenols are oxidized to alpha,omega-prenyl dicarboxylic acids which are catabolized from the omega-terminus, and (c) this metabolic process is not limited to insect cells.
D Gonzalez-Pacanowska; B Arison; C M Havel; J A Watson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  263     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1988 Jan 
Date Detail:
Created Date:  1988-02-25     Completed Date:  1988-02-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1301-6     Citation Subset:  IM    
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
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MeSH Terms
Cell Line
Drosophila / metabolism*
Farnesol / metabolism*
Liver / metabolism
Mevalonic Acid / metabolism
Polyisoprenyl Phosphates / metabolism
Rats, Inbred Strains
Grant Support
Reg. No./Substance:
0/Polyisoprenyl Phosphates; 0/Sesquiterpenes; 13058-04-3/farnesyl pyrophosphate; 150-97-0/Mevalonic Acid; 4602-84-0/Farnesol

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