| Isomaltitol exacerbates neutrophilia but reduces eosinophilia: new insights into the sephadex model of lung inflammation. | |
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MedLine Citation:
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PMID: 20962533 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: We have previously examined isomaltitol in an in vitro static adhesion assay and were interested in investigating whether the potentially anti-inflammatory effects observed there could be relevant in vivo. The Sephadex-induced lung inflammation model was considered a suitablemodel due to the significant changes in global inflammatory endpoints seen upon provocation with Sephadex. Methods: Male Sprague-Dawley rats were instilled intratracheally with Sephadex (5 mg/ml), vehicle (0.9% NaCl), isomaltitol (50 mg/ml) or a combination of isomaltitol and Sephadex. After 24 h, the lungs were weighed to measure edema and preserved for histology. Bronchoalveolar lavage fluid was used for analysis of tumor necrosis factor, cysteinyl leukotrienes, and differential and total leukocyte counts. Results: Differential counts showed that isomaltitol increased the neutrophil component while decreasing the eosinophilia, thus asserting a modulatory role on the usually eosinophil-dominated Sephadex-induced cell profile. Isomaltitol alone also increased edema and cysteinyl leukotrienes, and generally aggravated total inflammation in combination with Sephadex. The mechanisms were not investigated in this study, but effects could relate to a combination of isomaltitol's osmotic and structure-specific properties. Conclusion: Our results show that isomaltitol can modulate the inflammatory response induced by Sephadex instillation in addition to having proinflammatory effects on its own, and may therefore provide new insights into the mechanisms of this widely used animal model. Sugar alcohols similar to isomaltitol have already been used to aid mucus clearance in cystic fibrosis patients, and it is possible that isomaltitol could also be used for this purpose. |
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Authors:
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Chamilly Evaldsson; Ingvar Rydén; Srinivas Uppugunduri |
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Publication Detail:
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Type: Journal Article Date: 2010-10-20 |
Journal Detail:
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Title: International archives of allergy and immunology Volume: 154 ISSN: 1423-0097 ISO Abbreviation: Int. Arch. Allergy Immunol. Publication Date: 2011 |
Date Detail:
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Created Date: 2011-03-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9211652 Medline TA: Int Arch Allergy Immunol Country: Switzerland |
Other Details:
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Languages: eng Pagination: 286-94 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 S. Karger AG, Basel. |
Affiliation:
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Divisions of Cell Biology, Linköping University, Linköping, Sweden. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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