Document Detail


Isolation of the yeast phosphoglyceromutase gene and construction of deletion mutants.
MedLine Citation:
PMID:  3033435     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The PGM1 gene (also called GPM; Fraenkel 1982) coding for phosphoglyceromutase was isolated by functional complementation. When present on a multicopy vector and introduced into yeast cells it led to an about eightfold increase in specific enzymatic activity. This apparent overproduction was confirmed by SDS-polyacrylamide gel electrophoresis of crude extracts and at the transcriptional level by Northern analysis. By subcloning of the yeast DNA insertions of the plasmids originally isolated the PGM1 coding region was located within a 1.3 kb SalI-HindIII fragment. Integration at the chromosomal locus confirmed that the PGM1 gene had indeed been isolated. Southern analysis of genomic digests showed the same restriction patterns as the cloned sequences. However, a BamHI restriction polymorphism was observed. Furthermore, a repetitive element was found in the PGM1 flanking region. Finally, the chromosomal copy of the gene was deleted by replacement with a URA3 marker. The deletion mutants showed that the gene is not essential for yeast growing in the presence of a combination of glycerol and ethanol. However, growth was inhibited by glucose and neither glycerol nor ethanol alone were sufficient to support growth.
Authors:
R Rodicio; J Heinisch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  206     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-05-22     Completed Date:  1987-05-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  133-40     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromosome Deletion*
Cloning, Molecular*
DNA Restriction Enzymes
Genes*
Genes, Fungal*
Mutation*
Phosphoglycerate Mutase / genetics*
Phosphotransferases / genetics*
Plasmids
Saccharomyces cerevisiae / enzymology,  genetics*
Chemical
Reg. No./Substance:
EC 2.7.-/Phosphotransferases; EC 3.1.21.-/DNA Restriction Enzymes; EC 5.4.2.1/Phosphoglycerate Mutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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