Document Detail

Isolation of two discrete human interferon-gamma (immune) subtypes by high-performance liquid chromatography.
MedLine Citation:
PMID:  6203427     Owner:  NLM     Status:  MEDLINE    
A rapid procedure for isolation of two biologically active human interferon-gamma subtypes was developed. Crude interferon-gamma produced in a serum-free culture of peripheral blood mononuclear cells by mitogen stimulation was concentrated and partially purified by chromatography on controlled-pore glass. Following desalting and concentration by ultrafiltration, a step of cation-exchange high-performance liquid chromatography was performed. A linear NaCl gradient (0.01-0.4 M) at pH 7 was employed and four peaks of biological activity eluting at 0.17, 0.20, 0.26 (major peak), and 0.3 M were obtained. The major peak of biological activity coincided with two protein peaks. Analysis of one fraction from the major activity peak by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a protein band having an apparent molecular weight of 26,000, while an adjacent fraction of the same activity peak contained a protein band corresponding to a molecular weight of 21,000. The specific activity of both subtypes was 7-10 X 10(6) units/mg.
J Friedlander; D G Fischer; M Rubinstein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  137     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1984 Feb 
Date Detail:
Created Date:  1984-07-02     Completed Date:  1984-07-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  115-9     Citation Subset:  IM    
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MeSH Terms
Chromatography, High Pressure Liquid*
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Interferon-gamma / isolation & purification*
Molecular Weight
Staining and Labeling
Reg. No./Substance:
7440-22-4/Silver; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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