Document Detail


Isolation of two Pseudomonas strains producing pseudomonic acid A.
MedLine Citation:
PMID:  19070447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two novel Pseudomonas strains were isolated from groundwater sediment samples. The strains showed resistance against the antibiotics tetracycline, cephalothin, nisin, vancomycin, nalidixic acid, erythromycin, lincomycin, and penicillin and grew at temperatures between 15 and 37 degrees C and pH values from 4 to 10 with a maximum at pH 7 to 10. The 16S ribosomal RNA gene sequences and the substrate spectrum of the isolates revealed that the two strains belonged to the Pseudomonas fluorescens group. The supernatants of both strains had an antibiotic effect against Gram-positive bacteria and one Gram-negative strain. The effective substance was produced under standard cultivation conditions without special inducer molecules or special medium composition. The antibiotically active compound was identified as pseudomonic acid A by off-line high performance liquid chromatography (HPLC) and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). The measurement on ultra performance liquid chromatography (UPLC, UV-vis detection) confirmed the determination of pseudomonic acid A which was produced by both strains at 1.7-3.5mg/l. Our findings indicate that the ability to produce the antibiotic pseudomonic acid A (Mupirocin) is more spread among the pseudomonads then anticipated from the only producer known so far.
Authors:
Eva Fritz; Agnes Fekete; Jutta Lintelmann; Philipe Schmitt-Kopplin; Rainer U Meckenstock
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Publication Detail:
Type:  Journal Article     Date:  2008-12-12
Journal Detail:
Title:  Systematic and applied microbiology     Volume:  32     ISSN:  0723-2020     ISO Abbreviation:  Syst. Appl. Microbiol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-19     Completed Date:  2009-03-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306133     Medline TA:  Syst Appl Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  56-64     Citation Subset:  IM    
Affiliation:
Institute of Groundwater Ecology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU680856;  EU680857
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Drug Resistance, Bacterial
Fourier Analysis
Fresh Water / microbiology
Geologic Sediments / microbiology
Gram-Negative Bacteria / drug effects
Gram-Positive Bacteria / drug effects
Mass Spectrometry / methods
Microbial Sensitivity Tests
Molecular Sequence Data
Mupirocin / biosynthesis*,  chemistry,  pharmacology
Pseudomonas / classification*,  genetics,  isolation & purification*,  metabolism
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
12650-69-0/Mupirocin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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