Document Detail

Isolation of a tridecapeptide from bovine intestinal tissue and its partial characterization as neurotensin.
MedLine Citation:
PMID:  993204     Owner:  NLM     Status:  MEDLINE    
Radioimmunoassayable neurotensin (R-NT) has been isolated from acid/acetone extracts of 50 kg of calf small intestine with an overall yield of approximately 15%. The concentration of R-NT in calf intestinal tissue was approximately 35 pmol/g wet weight. Throughout the purification procedures which involved adsorption onto sulfopropyl (SP)-Sephadex, chromatography on Sephadex G-25 and SP-Sephadex, immunoadsorption on neurotensin-antibody Sepharose and high voltage paper electrophoresis, R-NT displayed the chromatographic and electrophoretic properties of neurotensin. R-NT was found to contain a tridecapeptide with the same amino acid composition as neurotensin. This peptide yielded the same products as neurotensin when submitted to digestion by carboxypeptidase A or papain. Its immunological properties were indistinguishable from those of neurotensin and its potency in stimulating hypotension in anesthetized rats was comparable to that of synthetic neurotensin. If the amino acid sequence of this peptide proves to be the same as that of neurotensin, then neurotensin is another biologically active peptide isolated from both brain and intestinal tissues.
P Kitabgi; R Carraway; S E Leeman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  251     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1976 Nov 
Date Detail:
Created Date:  1977-01-29     Completed Date:  1977-01-29     Revised Date:  2009-10-27    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7053-8     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis
Biological Assay
Blood Pressure / drug effects
Intestine, Small / analysis*
Kinins* / isolation & purification,  pharmacology
Peptides* / isolation & purification,  pharmacology
Reg. No./Substance:
0/Amino Acids; 0/Kinins; 0/Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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