Document Detail


Isolation of tetranucleotide repeat polymorphisms flanking the BRCA1 gene.
MedLine Citation:
PMID:  8786111     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Large pools of cosmids from the BRCA1 region of human chromosome 17 were screened for tetranucleotide repeat polymorphisms by hybridizing shotgun subcloned pools with a mixture of 25 oligonucleotides. Identified subclones were PCR amplified and directly sequenced to design PCR primers for short tandem repeat polymorphism (STRP) analysis of family DNAs. With the identification of the BRCA1 gene and the observation that most mutations in this > 100-kb gene are unique, haplotyping and linkage analysis may play a significant role in diagnosis and carrier detection of BRCA1-associated breast and ovarian cancers. We report the characterization of 15 new STRPs flanking the BRCA1 locus.
Authors:
P E Bennett-Baker; S Kiousis; S C Chandrasekharappa; S E King; K J Abel; F S Collins; B L Weber; J S Chamberlain
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genomics     Volume:  32     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-09-20     Completed Date:  1996-09-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  163-7     Citation Subset:  IM    
Affiliation:
Department of Human Genetics, Human Genome Center, University of Michigan Medical School, Ann Arbor 48109-0618, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U41191;  U41192;  U41193;  U41194;  U41195;  U41196;  U41197;  U41198;  U41199;  U41200;  U41201;  U41202;  U41203;  U41204;  U41205
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MeSH Terms
Descriptor/Qualifier:
BRCA1 Protein
Base Sequence
Breast Neoplasms / genetics
Chromosome Mapping
Chromosomes, Human, Pair 17 / genetics
Cloning, Molecular
Cosmids
DNA Primers / genetics
Female
Genes, Tumor Suppressor*
Humans
Microsatellite Repeats*
Molecular Sequence Data
Neoplasm Proteins / genetics*
Ovarian Neoplasms / genetics
Polymerase Chain Reaction
Polymorphism, Genetic*
Transcription Factors / genetics*
Grant Support
ID/Acronym/Agency:
HG00209/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/BRCA1 Protein; 0/DNA Primers; 0/Neoplasm Proteins; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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