Document Detail


Isolation and survey of novel fluoroacetate-degrading bacteria belonging to the phylum Synergistetes.
MedLine Citation:
PMID:  22372434     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Microbial dehalogenation of chlorinated compounds in anaerobic environments is well known, but degradation of fluorinated compounds under similar conditions has rarely been described. Here we report on the isolation of a bovine rumen bacterium that metabolizes fluoroacetate under anaerobic conditions, the mode of degradation and its presence in gut ecosystems. The bacterium was identified by 16S rRNA gene sequence analysis as belonging to the phylum Synergistetes and was designated strain MFA1. Growth was stimulated by amino acids with greater quantities of amino acids metabolized in the presence of fluoroacetate but sugars were not fermented. Acetate, formate, propionate, isobutryate, isovalerate, ornithine and H(2) were end-products of amino acid metabolism. Acetate was the primary end-product of fluoroacetate dehalogenation and the amount produced correlated with the stoichiometric release of fluoride which was confirmed using fluorine nuclear magnetic resonance ((19) F NMR) spectroscopy. Hydrogen and formate produced in situ were consumed during dehalogenation. The growth characteristics of strain MFA1 indicated that the bacterium may gain energy via reductive dehalogenation. This is the first study to identify a bacterium that can anaerobically dehalogenate fluoroacetate. Nested 16S rRNA gene specific PCR assays detected the bacterium at low numbers in the gut of several herbivore species. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Authors:
Carl K Davis; Richard I Webb; Lindsay I Sly; Stuart E Denman; Chris S McSweeney
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-28
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  -     ISSN:  1574-6941     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, 4067, QLD, Australia; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072, QLD, Australia.
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