Document Detail

Isolation, structure characterization and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense.
MedLine Citation:
PMID:  22500548     Owner:  NLM     Status:  Publisher    
A polysaccharide (GSP-6B) with a molecular weight of 1.86 ×106 Da was isolated from the fruiting bodies of Ganoderma sinense. Chemical composition analysis, methylation analysis, infra-red spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1→6)-linked-β-D-glucopyranosyl residues, bearing branches at O-3 position of every two sugar residues along the backbone. The side chains contain (1→4)-linked-β-D-glucopyranosyl residues, (1→3)-linked-β-D-glucopyranosyl residues and non-reducing end β-D-glucopyranosyl residues. In vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the releasing of IL-1β and TNF-in human PBMC and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate DC by stimulating the secretion of IL-12 and IL-10 from DC.
Xiao-Qiang Han; Ben Chung Lap Chan; Cai-Xia Dong; Yin-Hua Yang; Chun Hay Ko; Grace Gar-Lee Yue; Dan Chen; Chun-Kwok Wong; Clara Bik-San Lau; Pengfei Tu; Pang-Chui Shaw; Kwok-Pui Fung; Ping Chung Leung; Quan-Bin Han
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-13
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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