| Isolation and structural characterization of colistin components. | |
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MedLine Citation:
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PMID: 11560379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Preparative-scale separation of colistin sulphate bulk sample was carried out on a preparative poly(styrene-divinylbenzene) stationary phase. Isocratic elution with acetonitrile-sodium sulphate solution (0.7% m/v; pH adjusted to 2.5 with TFA) - water (16:50:34, % v/v/v) was carried out at a flow rate of 4.0 ml min(-1). Six colistin components were isolated and characterized using 1H and 13C NMR. The molecular weights were confirmed by mass spectrometry. The structures of 2 components were determined for the first time. Polymyxin E7 was identified as having the same composition as polymyxin E1, except that the fatty acid moiety was 7-methyloctanoic acid. Isoleucine polymyxin E8 was characterized as having the same composition as isoleucine polymyxin E1 with 7-methylnonanoic acid as the fatty acid moiety. |
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Authors:
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J A Orwa; C Govaerts; R Busson; E Roets; A Van Schepdael; J Hoogmartens |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of antibiotics Volume: 54 ISSN: 0021-8820 ISO Abbreviation: J. Antibiot. Publication Date: 2001 Jul |
Date Detail:
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Created Date: 2001-09-18 Completed Date: 2001-09-27 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0151115 Medline TA: J Antibiot (Tokyo) Country: Japan |
Other Details:
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Languages: eng Pagination: 595-9 Citation Subset: IM |
Affiliation:
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Katholieke Universiteit Leuven, Faculteit Farmaceutische Wetenschappen, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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chemistry*,
isolation & purification Carbon Isotopes Chromatography, High Pressure Liquid Colistin / chemistry*, isolation & purification Magnetic Resonance Spectroscopy |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Carbon Isotopes; 1066-17-7/Colistin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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