Document Detail

Isolation and structural characterization of colistin components.
MedLine Citation:
PMID:  11560379     Owner:  NLM     Status:  MEDLINE    
Preparative-scale separation of colistin sulphate bulk sample was carried out on a preparative poly(styrene-divinylbenzene) stationary phase. Isocratic elution with acetonitrile-sodium sulphate solution (0.7% m/v; pH adjusted to 2.5 with TFA) - water (16:50:34, % v/v/v) was carried out at a flow rate of 4.0 ml min(-1). Six colistin components were isolated and characterized using 1H and 13C NMR. The molecular weights were confirmed by mass spectrometry. The structures of 2 components were determined for the first time. Polymyxin E7 was identified as having the same composition as polymyxin E1, except that the fatty acid moiety was 7-methyloctanoic acid. Isoleucine polymyxin E8 was characterized as having the same composition as isoleucine polymyxin E1 with 7-methylnonanoic acid as the fatty acid moiety.
J A Orwa; C Govaerts; R Busson; E Roets; A Van Schepdael; J Hoogmartens
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of antibiotics     Volume:  54     ISSN:  0021-8820     ISO Abbreviation:  J. Antibiot.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-09-18     Completed Date:  2001-09-27     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0151115     Medline TA:  J Antibiot (Tokyo)     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  595-9     Citation Subset:  IM    
Katholieke Universiteit Leuven, Faculteit Farmaceutische Wetenschappen, Belgium.
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MeSH Terms
Anti-Bacterial Agents / chemistry*,  isolation & purification
Carbon Isotopes
Chromatography, High Pressure Liquid
Colistin / chemistry*,  isolation & purification
Magnetic Resonance Spectroscopy
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Carbon Isotopes; 1066-17-7/Colistin

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