Document Detail

Isolation and structural characterisation of rhamnogalacturonan oligomers generated by controlled acid hydrolysis of sugar-beet pulp.
MedLine Citation:
PMID:  9581279     Owner:  NLM     Status:  MEDLINE    
Controlled acid hydrolysis was applied to a deesterified beet pulp and the resulting soluble fraction was fractionated on a Biorad AG 1X8 column eluted by ammonium acetate pH 6 from 0.05 to 2 M. Eight retained fractions were obtained, containing almost exclusively GalA and Rha. Three types of oligomers could be identified: homogalacturonans, of which mono-,di- and tri-GalA were isolated as individual components, and two series of rhamnogalacturonan (RG) oligomers. One RG oligomer, isolated after ion-exchange chromatography, was identified as alpha-D-GalAp-(1-->2)-alpha-L-Rhap-(1-->4)-alpha-D-GalA p-(1-->2)-L-Rhap. The major peak contained oligomers of dp 6 to more than 20, of which dp 6 to 16 could be isolated on Bio-Gel P-6 + P-4. NMR of the oligomers of dp 6 to 10 showed the following structure: alpha-D-GalAp-(1[-->2)-alpha-L-Rhap-(1-->4)-alpha-D-GalA p-(1]n-->2)-L-Rhap. A second, quantitatively minor, series of RG oligomers eluted at higher ionic strength. These oligomers, which could be hydrolysed by RG-hydrolase and RG-lyase, were based on the alternating RG structure. Their non-reducing end was GalA, susceptible to hydrolysis by RG-galacturonohydrolase, and their reducing end might have more than one consecutive GalA.
C M Renard; M Lahaye; M Mutter; F G Voragen; J F Thibault
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Carbohydrate research     Volume:  305     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-06-02     Completed Date:  1998-06-02     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  271-80     Citation Subset:  IM    
Laboratoire de Biochimie et Technologie des Glucides, I.N.R.A., Nantes, France.
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MeSH Terms
Carbohydrate Sequence
Carbon Isotopes
Chenopodiaceae / chemistry*
Molecular Sequence Data
Nuclear Magnetic Resonance, Biomolecular
Oligosaccharides / chemistry*
Pectins / chemistry*
Reg. No./Substance:
0/Acids; 0/Carbon Isotopes; 0/Oligosaccharides; 0/Pectins; 0/Protons; 0/rhamnogalacturonan I

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