Document Detail

Isolation and some characterization of an acidic polysaccharide with anti-calcification activity from coccoliths of a marine alga, Pleurochrysis carterae.
MedLine Citation:
PMID:  11758934     Owner:  NLM     Status:  MEDLINE    
Coccolith is a calcified scale with species-specific fine structure produced by marine unicellular coccolithophorid algae, and consists of calcium carbonate crystals and organic matrices. EDTA-soluble organic materials extracted from coccoliths of Pleurochrysis carterae showed anti-calcification activity. They were separated by anion-exchange HPLC, and two fractions, fractions A and B, were obtained. Fraction B, which was more active than fraction A, was further separated into six consecutive fractions, B1-B6, by second anion-exchange HPLC. 1H NMR spectral analyses of these fractions suggested that a novel acidic polysaccharide, designated CMAP, existed throughout B1-B6 and that the latter four fractions mainly contained another acidic polysaccharide, PS-2, characterized previously. Since PS-2 did not show anti-calcification activity, CMAP was found to be the active principle.
N Ozaki; S Sakuda; H Nagasawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  65     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-12-07     Completed Date:  2002-05-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2330-3     Citation Subset:  IM    
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Japan.
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MeSH Terms
Algae / chemistry*,  metabolism
Calcification, Physiologic / drug effects*
Calcium Carbonate / chemistry,  metabolism
Chromatography, Ion Exchange
Hydrogen-Ion Concentration
Nuclear Magnetic Resonance, Biomolecular
Polysaccharides / chemistry,  isolation & purification*,  pharmacology*
Reg. No./Substance:
0/Polysaccharides; 471-34-1/Calcium Carbonate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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