Document Detail

Isolation and sequencing of CA/GT repeat microsatellites from chromosomal libraries without subcloning.
MedLine Citation:
PMID:  1595886     Owner:  NLM     Status:  MEDLINE    
A method for the isolation of (CA)n microsatellites from chromosome-specific genomic libraries is described. Clones were first screened using a polynucleotide CA/GT probe. Those shown to contain CA repeats were plaque purified and either subcloned or the insert amplified directly using vector primers. Polymerase chain reaction products were then used to directly sequence the regions flanking CA repeats by using biotinylated primers that amplify cloned inserts outward from CA repeat containing regions of DNA to vector primers. This method provides rapid access to microsatellites from chromosomes or chromosome regions of interest.
G R Taylor; S Haward; J S Noble; V Murday
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  200     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1992 Jan 
Date Detail:
Created Date:  1992-06-30     Completed Date:  1992-06-30     Revised Date:  2006-04-21    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  125-9     Citation Subset:  IM    
Yorkshire Regional DNA Laboratory, Leeds, England.
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MeSH Terms
Base Sequence
Chromosomes, Human, Pair 21*
Cloning, Molecular
DNA, Satellite / genetics*
Genetic Vectors
Hybrid Cells
Molecular Sequence Data
Oligonucleotides / genetics
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid
Reg. No./Substance:
0/DNA, Satellite; 0/Oligonucleotides

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