| Isolation and sequencing of a cDNA clone encoding acid phosphatase in rat liver lysosomes. | |
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MedLine Citation:
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PMID: 2764916 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A full length cDNA for acid phosphatase in rat liver lysosomes was isolated and sequenced. The predicted amino acid sequence comprises 423 residues (48,332 Da). A putative signal peptide of 30 residues is followed by the NH2-terminal sequence of lysosomal acid phosphatase (45,096 Da). The deduced NH2-terminal 18-residue sequence is identical with that determined directly for acid phosphatases purified from the rat liver lysosomal membranes. The primary structure deduced for acid phosphatase contains 9 potential N-glycosylation sites and a hydrophobic region which could function as a transmembrane domain. It exhibits 89% and 67% sequence similarities in amino acids and nucleic acids, respectively, to human lysosomal acid phosphatase. The amino acid sequence of the putative transmembrane segment shows a complete similarity to that of the human enzyme. Northern blot hybridization analysis identified a single species of acid phosphatase mRNA (2.2 kbp in length) in rat liver. |
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Authors:
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M Himeno; H Fujita; Y Noguchi; A Kono; K Kato |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 162 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1989 Aug |
Date Detail:
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Created Date: 1989-09-21 Completed Date: 1989-09-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1044-53 Citation Subset: IM |
Affiliation:
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Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/M27893 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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genetics* Amino Acid Sequence Animals Base Sequence Blotting, Northern Cloning, Molecular DNA / genetics Liver / enzymology Lysosomes / enzymology Membrane Proteins / genetics, ultrastructure Molecular Sequence Data Rats Restriction Mapping |
| Chemical | |
Reg. No./Substance:
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0/Membrane Proteins; 9007-49-2/DNA; EC 3.1.3.2/Acid Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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