Document Detail

Isolation and sequencing of a cDNA clone encoding acid phosphatase in rat liver lysosomes.
MedLine Citation:
PMID:  2764916     Owner:  NLM     Status:  MEDLINE    
A full length cDNA for acid phosphatase in rat liver lysosomes was isolated and sequenced. The predicted amino acid sequence comprises 423 residues (48,332 Da). A putative signal peptide of 30 residues is followed by the NH2-terminal sequence of lysosomal acid phosphatase (45,096 Da). The deduced NH2-terminal 18-residue sequence is identical with that determined directly for acid phosphatases purified from the rat liver lysosomal membranes. The primary structure deduced for acid phosphatase contains 9 potential N-glycosylation sites and a hydrophobic region which could function as a transmembrane domain. It exhibits 89% and 67% sequence similarities in amino acids and nucleic acids, respectively, to human lysosomal acid phosphatase. The amino acid sequence of the putative transmembrane segment shows a complete similarity to that of the human enzyme. Northern blot hybridization analysis identified a single species of acid phosphatase mRNA (2.2 kbp in length) in rat liver.
M Himeno; H Fujita; Y Noguchi; A Kono; K Kato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  162     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-09-21     Completed Date:  1989-09-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1044-53     Citation Subset:  IM    
Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
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MeSH Terms
Acid Phosphatase / genetics*
Amino Acid Sequence
Base Sequence
Blotting, Northern
Cloning, Molecular
DNA / genetics
Liver / enzymology
Lysosomes / enzymology
Membrane Proteins / genetics,  ultrastructure
Molecular Sequence Data
Restriction Mapping
Reg. No./Substance:
0/Membrane Proteins; 9007-49-2/DNA; EC Phosphatase

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