Document Detail

Isolation and sequence of the t-RNA ligase-encoding gene of Candida albicans.
MedLine Citation:
PMID:  8181747     Owner:  NLM     Status:  MEDLINE    
The gene encoding tRNA ligase from Candida albicans was isolated from a genomic library by complementation of a Saccharomyces cerevisiae strain containing a disrupted structural gene, RLG1, encoding tRNA ligase. The cloned gene also complements a temperature-sensitive allele of RLG1. Sequence analysis revealed a single 2499-nt coding region. The gene encodes a protein of 833 amino acids that is 42% identical to S. cerevisiae tRNA ligase. Hybridization to chromosomes of C. albicans separated by pulsed-field gel electrophoresis located the gene to chromosome 1, the smallest C. albicans chromosome.
J Baymiller; S Jennings; B Kienzle; J A Gorman; R Kelly; J E McCullough
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gene     Volume:  142     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-06-10     Completed Date:  1994-06-10     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  129-34     Citation Subset:  IM    
Department of Microbial Molecular Biology, Bristol Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Candida albicans / enzymology,  genetics*
Chromosomes, Fungal
DNA, Fungal / isolation & purification
Genetic Complementation Test
Molecular Sequence Data
RNA Ligase (ATP) / genetics*
Restriction Mapping
Reg. No./Substance:
0/DNA, Fungal; EC Ligase (ATP)

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