Document Detail

Isolation and sequence of a cDNA clone for human calcineurin B, the Ca2+-binding subunit of the Ca2+/calmodulin-stimulated protein phosphatase.
MedLine Citation:
PMID:  2558868     Owner:  NLM     Status:  MEDLINE    
We have identified and cloned human cDNA for the Ca2+-binding subunit of calcineurin, the brain isozyme of the Ca2+/calmodulin-stimulated protein phosphatase. The 2.5-kb cDNA has an open reading frame of 510 bp, a leader sequence of at least 500 bp, and a 1,277-bp 3'-noncoding sequence. The deduced sequence of the human protein differs from bovine brain calcineurin B by an additional valine at the carboxyl terminus and substitution of Met-11 and Ser-153 by cysteine. A partial clone of the mouse protein corresponding to amino acids 75-150 was also isolated. This portion of the human and mouse protein sequence is identical, with the DNA sequences showing 94% identity. The respective mRNAs in human and mouse are also of similar size. As was observed with protein levels, mRNA abundance in brain is 20-60 times that found in other tissues with the exception of HeLa cells which, like brain, contain abundant calcineurin B mRNA.
D Guerini; M H Krinks; J M Sikela; W E Hahn; C B Klee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  DNA (Mary Ann Liebert, Inc.)     Volume:  8     ISSN:  0198-0238     ISO Abbreviation:  DNA     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-03-06     Completed Date:  1990-03-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8302432     Medline TA:  DNA     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  675-82     Citation Subset:  IM    
Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Calcium / physiology
Calmodulin-Binding Proteins / genetics*,  metabolism
Cloning, Molecular
DNA Probes
Escherichia coli / genetics
Molecular Sequence Data
Phosphoprotein Phosphatases / genetics*,  metabolism
RNA, Messenger / biosynthesis
Recombinant Proteins / metabolism
Sequence Homology, Nucleic Acid
Reg. No./Substance:
0/Calmodulin-Binding Proteins; 0/DNA Probes; 0/RNA, Messenger; 0/Recombinant Proteins; 7440-70-2/Calcium; EC; EC Phosphatases

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