Document Detail


Isolation and sequence of a cDNA clone for human calcineurin B, the Ca2+-binding subunit of the Ca2+/calmodulin-stimulated protein phosphatase.
MedLine Citation:
PMID:  2558868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have identified and cloned human cDNA for the Ca2+-binding subunit of calcineurin, the brain isozyme of the Ca2+/calmodulin-stimulated protein phosphatase. The 2.5-kb cDNA has an open reading frame of 510 bp, a leader sequence of at least 500 bp, and a 1,277-bp 3'-noncoding sequence. The deduced sequence of the human protein differs from bovine brain calcineurin B by an additional valine at the carboxyl terminus and substitution of Met-11 and Ser-153 by cysteine. A partial clone of the mouse protein corresponding to amino acids 75-150 was also isolated. This portion of the human and mouse protein sequence is identical, with the DNA sequences showing 94% identity. The respective mRNAs in human and mouse are also of similar size. As was observed with protein levels, mRNA abundance in brain is 20-60 times that found in other tissues with the exception of HeLa cells which, like brain, contain abundant calcineurin B mRNA.
Authors:
D Guerini; M H Krinks; J M Sikela; W E Hahn; C B Klee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  DNA (Mary Ann Liebert, Inc.)     Volume:  8     ISSN:  0198-0238     ISO Abbreviation:  DNA     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-03-06     Completed Date:  1990-03-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8302432     Medline TA:  DNA     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  675-82     Citation Subset:  IM    
Affiliation:
Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M30773
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Calcineurin
Calcium / physiology
Calmodulin-Binding Proteins / genetics*,  metabolism
Cattle
Cloning, Molecular
DNA Probes
Escherichia coli / genetics
Humans
Mice
Molecular Sequence Data
Mutation
Phosphoprotein Phosphatases / genetics*,  metabolism
RNA, Messenger / biosynthesis
Rabbits
Recombinant Proteins / metabolism
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
0/Calmodulin-Binding Proteins; 0/DNA Probes; 0/RNA, Messenger; 0/Recombinant Proteins; 7440-70-2/Calcium; EC 3.1.3.16/Calcineurin; EC 3.1.3.16/Phosphoprotein Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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