Document Detail


Isolation and sequence analysis of canine respiratory coronavirus.
MedLine Citation:
PMID:  17092595     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Canine respiratory coronavirus (CRCoV) has frequently been detected in respiratory samples from dogs by RT-PCR. In this report the first successful isolation of CRCoV from a dog with respiratory disease is described. The isolate CRCoV-4182 was cultured in HRT-18 cells but failed to replicate in a number of other cell lines. The nucleotide sequence of the 3'-terminal portion of the CRCoV genome was determined including all open reading frames from the NS2 gene to the N gene. Comparison with other coronavirus sequences showed a high similarity to bovine coronavirus (BCoV). The region between the spike and the E gene was found to be the most variable and was used for phylogenetic analysis of several CRCoV strains. CRCoV-4182 showed a mutation within the non-structural protein region downstream of the S gene leading to the translation of an 8.8 kDa putative protein comprising a fusion of the equivalent of the BCoV 4.9 kDa protein to a truncated version of the BCoV 4.8 kDa protein. The culture of CRCoV will enable analysis of the expression and function of this and other CRCoV proteins as well as allowing the study of the role of CRCoV in the aetiology of canine infectious respiratory disease.
Authors:
Kerstin Erles; Kai-Biu Shiu; Joe Brownlie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-07
Journal Detail:
Title:  Virus research     Volume:  124     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-13     Completed Date:  2007-09-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  78-87     Citation Subset:  IM    
Affiliation:
The Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, United Kingdom. kerles@rvc.ac.uk
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ682406
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Cercopithecus aethiops
Coronavirus, Bovine / genetics
Coronavirus, Canine / classification,  genetics*,  isolation & purification*
Cricetinae
Cricetulus
Dog Diseases / virology
Dogs
Genome, Viral*
Molecular Sequence Data
Mutation
Phylogeny
Polymorphism, Genetic
RNA, Viral / genetics*
Respiratory Tract Infections / veterinary,  virology
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology
Viral Proteins / genetics
Chemical
Reg. No./Substance:
0/RNA, Viral; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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