| Isolation and sequence analysis of canine respiratory coronavirus. | |
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MedLine Citation:
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PMID: 17092595 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Canine respiratory coronavirus (CRCoV) has frequently been detected in respiratory samples from dogs by RT-PCR. In this report the first successful isolation of CRCoV from a dog with respiratory disease is described. The isolate CRCoV-4182 was cultured in HRT-18 cells but failed to replicate in a number of other cell lines. The nucleotide sequence of the 3'-terminal portion of the CRCoV genome was determined including all open reading frames from the NS2 gene to the N gene. Comparison with other coronavirus sequences showed a high similarity to bovine coronavirus (BCoV). The region between the spike and the E gene was found to be the most variable and was used for phylogenetic analysis of several CRCoV strains. CRCoV-4182 showed a mutation within the non-structural protein region downstream of the S gene leading to the translation of an 8.8 kDa putative protein comprising a fusion of the equivalent of the BCoV 4.9 kDa protein to a truncated version of the BCoV 4.8 kDa protein. The culture of CRCoV will enable analysis of the expression and function of this and other CRCoV proteins as well as allowing the study of the role of CRCoV in the aetiology of canine infectious respiratory disease. |
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Authors:
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Kerstin Erles; Kai-Biu Shiu; Joe Brownlie |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-11-07 |
Journal Detail:
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Title: Virus research Volume: 124 ISSN: 0168-1702 ISO Abbreviation: Virus Res. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-13 Completed Date: 2007-09-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8410979 Medline TA: Virus Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 78-87 Citation Subset: IM |
Affiliation:
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The Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, United Kingdom. kerles@rvc.ac.uk |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/DQ682406 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cell Line Cercopithecus aethiops Coronavirus, Bovine / genetics Coronavirus, Canine / classification, genetics*, isolation & purification* Cricetinae Cricetulus Dog Diseases / virology Dogs Genome, Viral* Molecular Sequence Data Mutation Phylogeny Polymorphism, Genetic RNA, Viral / genetics* Respiratory Tract Infections / veterinary, virology Sequence Alignment Sequence Analysis, DNA Sequence Homology Viral Proteins / genetics |
| Chemical | |
Reg. No./Substance:
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0/RNA, Viral; 0/Viral Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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