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Isolation and separation of highly enriched fractions of viable mouse gastric parietal cells by velocity sedimentation.
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MedLine Citation:
PMID:  1127018     Owner:  NLM     Status:  MEDLINE    
Methods of tissue dissociation and cell separation have been modified to obtain highly enriched fractions of mouse gastric parietal cells. Suspension of gastric mucosal cells are prepared by pronase digestion of the glandular portion of the stomach from adult mice. By utilizing the velocity sedimentation technique to separate cells of different sizes it is possible to recovery parietal cells, which are larger than the other cell types, in fractions with purity of 75-95%. The homogeneity of cell fractions has been assessed by light and electron microscopy. The ability of the isolated cells to exclude the dye trypan blue, to incorporate labeled substrate, to consume oxygen, and to retain their structural integrity indicates that they are viable and still capable of functional activity.
L J Romrell; M R Coppe; D R Munro; S Ito
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  65     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1975 May 
Date Detail:
Created Date:  1975-07-23     Completed Date:  1975-07-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  428-38     Citation Subset:  IM    
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MeSH Terms
Cell Separation / methods*
Centrifugation, Density Gradient
Cytoplasmic Granules / ultrastructure
Gastric Mucosa / cytology*,  ultrastructure
Microscopy, Electron
Microscopy, Interference
Organoids / ultrastructure
Oxygen Consumption
Pronase / metabolism
Reg. No./Substance:
EC 3.4.24.-/Pronase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 5 Year: 1975
Volume: 65 Issue: 2
First Page: 428 Last Page: 438
ID: 2109418
Publisher Id: 75151746
PubMed Id: 1127018

Isolation and separation of highly enriched fractions of viable mouse gastric parietal cells by velocity sedimentation

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