Document Detail

Isolation and seasonal effects on characteristics of fulvic acid isolated from an Australian floodplain river and billabong.
MedLine Citation:
PMID:  17010354     Owner:  NLM     Status:  MEDLINE    
Fulvic acids from an Australian floodplain river and billabong were isolated using DEAE and DAX-8 resins, and characterised with the use of size exclusion chromatography and solid-state CP-MAS (13)C NMR spectroscopy. Differences between the two resin isolates were evident. Fulvic acids isolated using DEAE-cellulose had higher apparent M(n) and M(w) values, while the DAX-8 resin showed a slight preference for aliphatic components. Fulvic acids from the river and billabong had the same functional groups present, however, the river fulvic acids had higher apparent M(n) (number average molecular weight) and M(w) values (weight average molecular weight), and were more polydisperse than the billabong fulvic acid. There were no significant changes in the characteristics of the fulvic acid isolated from the river at four sampling times: summer, autumn, winter and spring. In contrast, fulvic acids isolated from a billabong displayed seasonal variation in molecular weights. This work emphasizes the importance in ecological studies of isolation procedure for the operationally defined fulvic acids.
Suzanne McDonald; Jennifer M Pringle; Andrea G Bishop; Paul D Prenzler; Kevin Robards
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-28
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1153     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-21     Completed Date:  2007-07-25     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  203-13     Citation Subset:  IM    
School of Science and Technology, Charles Sturt University, P.O. Box 588, Wagga Wagga 2678, NSW, Australia.
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MeSH Terms
Benzopyrans / chemistry,  isolation & purification*
Chromatography, Gel
Chromatography, High Pressure Liquid
Humic Substances / analysis*
Molecular Weight
Nuclear Magnetic Resonance, Biomolecular
Water Pollutants, Chemical / chemistry,  isolation & purification*
Reg. No./Substance:
0/Benzopyrans; 0/Humic Substances; 0/Water Pollutants, Chemical; 479-66-3/fulvic acid

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